1.13306

Bactident^™ Coagulase

For the detection of the enzyme coagulase developed by Staphylococcus aureus

• Synonym(s): Test on Staphylococcus aureus, Test on Coagulase
• UNSPSC Code: 41116130
• NACRES: NA.84

Properties

• material: tube
• Quality Level: 100
• form: solid
• feature: ready-to-use
• packaging: pack of 6 vials with 3 mL volume
• manufacturer/tradename: Bactident^™
• storage condition: (Store dry and tightly closed)
• technique(s): microbe id | specific enzyme detection: suitable
• color: beige
• application(s): food and beverages
• storage temp.: 2-8°C
• suitability: Staphylococcus aureus; Staphylococcus spp. (spp.)

Description

General description

Bactident^™ Coagulase reagent enables reliable detection of the enzyme coagulase developed by Staphylococcus aureus. Coagulase is an enzyme with the ability to coagulate plasma. Staphylococcus aureus forms two types of coagulases; (i) the free coagulase is an extracellular enzyme, and (ii) the bound coagulase is localized on the surface of the cell wall. The tube test can detect both enzymes, while the slide test can detect only the bound coagulase.

Application

An overnight broth culture of Staphylococcus aureus is incubated with rehydrated EDTA-rabbit plasma, which is contained in Bactident^™ Coagulase. The coagulase test is positive if more than three-quarters of the tube contents form a coherent clot.

Analysis Note

Coagulase
- Staphylococcus aureus ATCC 25923 (WDCM 00034):
+
- Staphylococcus epidermidis ATCC 12228 (WDCM 00036):-
- Negative control:-

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2