Skip to Content
Merck

21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

CFSE

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

Pricing and availability is not currently available.

About This Item

Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
150347-59-4
Molecular Weight:
557.46
NACRES:
NA.32
PubChem Substance ID:
57647951
UNSPSC Code:
12352108
MDL number:
MFCD00037466

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, BioReagent, suitable for fluorescence, ≥90% (HPLC)

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Quality Level

200

description

suitable as fluorogenic substrate for esterases

product line

BioReagent

assay

≥90% (HPLC)

form

solid

mol wt

557.46 g/mol

color

almost white to yellow

mp

152-154 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
218842187921878
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sigma-Aldrich

21888

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

Sigma-Aldrich

21884

5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

5(6)-Carboxyfluorescein diacetate BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Sigma-Aldrich

21879

5(6)-Carboxyfluorescein diacetate

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Sigma-Aldrich

21878

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 492 nm; λem 517 nm in DMF

form

solid

form

-

form

powder

form

powder

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

solubility

DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[1][2] Forms green fluorescent conjugates on deacetylation.
5[3]-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry[4][5]
  • in lymphocyte proliferation assay[6][7]
  • to determine the intracellular or intra-organellar pH in chloroplasts[3]
  • to label Leishmania amazonensis and Leishmania major[8]

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.[9]

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.[10]

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCK2345
BCCF7480
BCCC4532
BCBW9190
BCBT5235

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
Microbial Food Safety Along the Dairy Chain (2017)
Generation and characterization of CD1d-specific single-domain antibodies with distinct functional features
Lameris R, et al.
Immunology, 149(1), 111-121 (2016)
View All Related Papers

Questions

1–6 of 6 Questions  

  1. What is the in vivo (mouse) half-life of CFDA-SE? What is the route of clearance (kidney, liver?)

    1 answer

    1. The half-life is not determined for this product.
      Please see the link below to a publication that may be helpful:
      https://www.semanticscholar.org/paper/The-Use-of-Carboxyfluorescein-Diacetate-Ester-to-Quah-Parish/cae3435e063cb9f46cea4ddfc7c560f5821e19b3

      Please note that, unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Helpful?

  2. At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?

    1 answer

    1. We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.

      Helpful?

  3. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?

    1 answer

    1. No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.

      Helpful?

  4. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?

    1 answer

    1. Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?

    1 answer

    1. Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service