D4913

2′-Deoxycytidine 5′-triphosphate disodium salt

100 mM (pH 7)

• Synonym(s): 2′-Deoxycytidine-5′-triphosphoric acid disodium salt, dCTP-Na₂
• Empirical Formula (Hill Notation): C₉H₁₄N₃Na₂O₁₃P₃
• CAS Number: 102783-51-7
• Molecular Weight: 511.12
• MDL number: MFCD00084683
• UNSPSC Code: 41106305
• PubChem Substance ID: 24893907
• NACRES: NA.52

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• Assay: ≥99%
• form: liquid
• concentration: 100 mM (pH 7)
• color: colorless
• foreign activity: DNase, RNase, none detected
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChI key: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Description

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is a cytidine analog.[1] It is composed of a nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar bound to a chain of three phosphate residues. dCTP is utilized by cells for DNA polymerase catalyzed synthesis of DNA.[2]

Application

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:

• in reverse transcription polymerase chain reaction (RT -PCR).[3]
• to study the mutations in HBB gene via amplification refractory mutation system (ARMS)-PCR[4]
• to C-tail the end-repaired DNA[5]

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves