I2393

IL-1β rat

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-1β, Interleukin-1β from rat, Lymphocyte activating factor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD01094098

Properties

• Product Name: Interleukin-1β from rat, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: rat
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• potency: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• mol wt: 17.3 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg
• UniProt accession no.: Q63264
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: rat ... Il1b(24494)

Description

Analysis Note

The biological activity is measured in a cell proliferation assay using murine D10S cells.

General description

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.¹ IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,² and is thought to play a key role in inflammatory and immune responses.³ Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRF_H), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).⁴ The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)