E6779

EZview^™ Red Anti-HA Affinity Gel

• Synonym(s): Anti-HA
• NACRES: NA.56
• UNSPSC Code: 12352203
• Technique(s): affinity chromatography: suitable, immunoprecipitation (IP): suitable
• Species reactivity: -
• Application: IP, affinity chromatography
• Citations: 242

Properties

• shelf life: 1 yr at 2‑8 °C
• Quality Segment: 200
• analyte chemical class(es): proteins (hemagglutinin)
• technique(s): affinity chromatography: suitable, immunoprecipitation (IP): suitable
• matrix: crosslinked agarose
• capacity: ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)
• storage temp.: 2-8°C

Description

General description

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Application

EZview^™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay.

Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.

When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview^™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Biochem/physiol Actions

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Features and Benefits

• Increased visibility - Red color reduces risk of incidental aspiration
• Improved recovery of target protein by reduced accidental loss
• Higher reproducibility - More consistent yields

Physical form

1:1 (v/v) suspension in PBS containing 50% glycerol and MIT-HClis present at 30 PPM and 0.003% (w/v).

Other Notes

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable