LSKMAGS08
PureProteome Magnetic Stand
The PureProteome Magnetic Stand is designed to rapidly & easily isolate magnetic particles from up to eight 1. 5 mL or 2. 0 mL tubes.
Synonym(s):
Magnetic Bead Stand, Magnetic Separation Stand
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About This Item
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material
self-standing
feature
binder
manufacturer/tradename
PureProteome
technique(s)
RNA purification: suitable (with magnetic beads)
protein purification: suitable
shipped in
ambient
Related Categories
General description
Application
Cell Culture
Features and Benefits
- Enables reproducible process
- Comparable results with standard protocols
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Certificates of Analysis (COA)
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Articles
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
PureProteome™ Protein A and G Magnetic beads provide a rapid and reproducible means to purify immunoglobulins (IgG) using the KingFisher Duo particle processor.
Related Content
Immunoprecipitation (IP) is a powerful technique for proteomic screening, biomarker discovery, and signaling network elucidation. It is frequently used to enrich target proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A, Protein G or a mixture of Protein A and G coupled to a solid support resin, such as agarose beads, to capture an antigen/antibody complex in solution. As the number of samples increase, the traditional, manual IP method can be time-consuming. Processing of multiple IP reactions in parallel can introduce complexity, variability and pipetting errors, which may affect reproducibility.
Biomarkers offer important information about homeostasis, disease, response to drug treatments, and environmental stimuli. Sera are rich sources of biomarkers (biological indicator proteins, peptides, small molecules, etc.) and are easier to sample than other tissues. However, the complexity of serum and the presence of highly abundant proteins like albumin and immunoglobulin can mask less abundant species, hindering biomarker detection. PureProteome albumin magnetic beads remove more than 98% of albumin from human serum. Here, we demonstrate that PureProteome albumin magnetic beads may also be used to remove albumin from mouse, guinea pig and rat sera. Depleted samples are often dilute, and may need concentration for downstream analyses. Therefore, we present a protocol for the convenient concentration of these samples using Amicon Ultra 2 mL centrifugal filters.
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