81845

Propidium iodide

≥94% (HPLC), apoptosis marker, powder

• Synonym(s): 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
• Empirical Formula (Hill Notation): C₂₇H₃₄I₂N₄
• CAS Number: 25535-16-4
• Molecular Weight: 668.39
• NACRES: NA.77
• PubChem Substance ID: 329769221
• UNSPSC Code: 12352200
• EC Number: 247-081-0
• MDL number: MFCD00011921
• Beilstein/REAXYS Number: 3843838

Properties

• Product Name: Propidium iodide, ≥94% (HPLC)
• InChI key: XJMOSONTPMZWPB-UHFFFAOYSA-M
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• SMILES string: [I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• assay: ≥94% (HPLC)
• form: powder
• mp: 220-225 °C (dec.) (lit.)
• fluorescence: λ_ex 482 nm; λ_em 608 nm (pH 7.2); λ_ex 540 nm; λ_em 608 nm (bound to DNA)
• storage temp.: 2-8°C
• Quality Level: 200

Description

Application

Propidium iodide has been used:

• to stain DNA in cell cycle analysis[1]
• to stain cells[2] and cell nuclei[3][4]
• for the preparation of propidium iodide (PI)stock solution[5]
• to stain cells for flow cytometry[6][7]

in scrape-loading dye transfer assay[8]

Features and Benefits

This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

General description

PI is used for selective labelling of dead cells.[9]

Propidium iodide is a fluorescent intercalating agent used for staining.[10] It functions as an apoptosis marker[11] and has linear DNA-binding capacity.[12] Propidium iodide is an effective indicator of tissue injury.[13]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H341
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 2
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves