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Merck

21115

Calcium chloride solution

BioUltra, Molecular Biology, ~1 M in H2O

Calcium chloride solution

Synonym(s):

CaCl2, Calcium chloride hydrate, Calcium dichloride

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About This Item

Empirical Formula (Hill Notation):
CaCl2
CAS Number:
Molecular Weight:
110.98
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25
Grade:
Molecular Biology
Form:
liquid

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grade

Molecular Biology

product line

BioUltra

form

liquid

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.358

pH

4.0-6.0 (25 °C)

density

1.086 g/mL at 20 °C

cation traces

Ba: ≤1 mg/kg
Bi: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mg: ≤5 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤20 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

SMILES string

Cl[Ca]Cl

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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Show Differences

1 of 4

This Item
902179C1016793639
form

liquid

form

granular

form

granular

form

powder

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

100

grade

for molecular biology

grade

anhydrous

grade

anhydrous

grade

ACS reagent, anhydrous

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, phosphatases, none detected

impurities

-

impurities

-

impurities

≤0.006 meq/g Titr. base

density

1.086 g/mL at 20 °C

density

-

density

-

density

-

pH

4.0-6.0 (25 °C)

pH

-

pH

-

pH

-

Application

Calcium chloride solution has been used in hippocampal slice culture preparation[1] and HEPES-buffered tyrode′s solution.[2]

Other Notes

In electric-field induced DNA transfection - increased by millimolar concentrations of calcium chloride[3].

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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T D Xie et al.
Biophysical journal, 58(1), 13-19 (1990-07-01)
A study of mechanisms of electrotransfection using Escherichia coli (JM 105) and the plasmid DNA pBR322 as model system is reported. pBR322 DNA carries an ampicillin resistance gene: E. coli transformants are conveniently assayed by counting colonies in a selection
Fluid osmolarity acutely and differentially modulates lymphatic vessels intrinsic contractions and lymph flow
Solari E, et al.
Frontiers in Physiology, 871-871 (2018)
Insulin-like growth factor-1 abrogates microglial oxidative stress and TNF-alpha responses to spreading depression
Grinberg Y Y, et al.
Journal of Neurochemistry, 126(5), 662-672 (2013)
J Philippe et al.
International journal of obesity (2005), 39(2), 295-302 (2014-06-04)
A significant proportion of severe familial forms of obesity remain genetically elusive. Taking advantage of our unique cohort of multigenerational obese families, we aimed to assess the contribution of rare mutations in 29 common obesity-associated genes to familial obesity, and
Michael A Goren et al.
Nature communications, 5, 5115-5115 (2014-10-09)
Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that scrambling is a constitutive activity of

Protocols

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Questions

1–8 of 8 Questions  
  1. is it sterile?

    1 answer
    1. This product is a calcium chloride solution for molecular biology; however, it is not sterile. Product C-34006 is a sterile calcium chloride solution.

      Helpful?

  2. Is this material classified as endotoxin free?

    1 answer
    1. This product is not tested for Endotoxin. Currently, there is only 1 Calcium Chloride product that is tested for Endotoxin, product 1371015000. This is a powder form with a maximum Endotoxin specification of 2.5 I.U./gram. Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sigma/21115

      Helpful?

  3. What is the purity percentage?

    1 answer
    1. Purity testing is not performed on this solution. Testing includes trace analysis and verification of the concentration. However, the Calcium Chloride used to prepare this solution has a minimum purity of 99.5%.

      Helpful?

  4. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  5. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  6. How long is this stable at room temperature in an unopened state?

    1 answer
    1. This product may be stored in room temperature. Concerning expiration, this product is assigned a product retest date. Products that, while considered stable, may be subject to degradation over time are assigned a periodic retest date to confirm quality. The lot-specific retest date is reported on the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/21115#product-documentation

      Helpful?

  7. what is the storage temperature for this?

    1 answer
    1. This product is stored tightly closed at room temperature.

      Helpful?

  8. Is this suitable for cell culture?

    1 answer
    1. The item is tested for suitability in molecular biology applications. It has not been tested for use in cell culture. Please see the link below to review product C-34006, Calcium Chloride Solution, which is suitable for cell culture use:
      https://www.sigmaaldrich.com/product/sigma/c34006

      Helpful?

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