62310

Lipase from Rhizopus niveus

powder (fine), ≥1.5 U/mg

• Synonym(s): Triacylglycerol acylhydrolase, Triacylglycerol lipase
• CAS Number: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.26
• EC Number: 232-619-9
• EC Number: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509

Properties

• Product Name: Lipase from Rhizopus niveus, powder (fine), ≥1.5 U/mg
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• biological source: fungus (Rhizopus niveus)
• form: powder (fine)
• specific activity: ≥1.5 U/mg
• mol wt: M_r ~83000
• storage temp.: 2-8°C
• Quality Level: 100

Description

Application

Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.

Biochem/physiol Actions

Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

General description

Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol fatty acid from a triglyceride per minute at pH 7.7 and 40°C (olive oil as substrate)]; 300 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314, at pH 8.0 and 40°C as substrate.

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Substrate specificity: preferentially hydrolyzes the 1- and 3-positions(middle to long fatty acids); optimum pH 5.0-7.0; optimum temperature 30-45°C; Selective enzymatic removal of protecting functions (C-terminal COOH function)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)