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Merck

C4859

Cycloheximide solution

Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

Synonym(s):

CHX

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About This Item

Empirical Formula (Hill Notation):
C15H23NO4
CAS Number:
66-81-9
Molecular Weight:
281.35
NACRES:
NA.77
PubChem Substance ID:
24892732
UNSPSC Code:
12352111
MDL number:
MFCD00082346

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Product Name

Cycloheximide solution, Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

InChI

1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1

SMILES string

O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C

InChI key

YPHMISFOHDHNIV-FSZOTQKASA-N

biological source

microbial

description

suitable for testing specifications (CHO C. growth inhibitor)

form

ready-to-use solution

concentration

100 mg/mL in DMSO

solubility

DMSO: soluble

antibiotic activity spectrum

fungi
yeast

mode of action

protein synthesis | interferes

storage temp.

2-8°C

Quality Level

300

Gene Information

human ... FKBP1A(2280), PIN1(5300)

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Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.
Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.

Application

Cycloheximide solution has been used:
  • to study its role in modulating cell death induced by tumor necrosis factor α (TNFα) in combination with the second mitochondria-derived activator of caspases (SMAC) mimetic birinapant or interferon-gamma (IFNγ), specifically under conditions that inhibit caspase-8 activity
  • in the study to inhibit protein translation during the CHX-chase assays
  • to treat antisense oligonucleotide (AON) transfected patient-derived fibroblasts and inhibit the nonsense-mediated decay (NMD) pathway to analyze the therapeutic potential AON and visualize the transcript containing the pseudoexon (PE)

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
0000486680
0000486680
0000471003
0000471003
0000431728

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Proceedings of the National Academy of Sciences of the United States of America, 111(43), 15438-15443 (2014-10-16)
The receptor-interacting kinase-3 (RIP3) and its downstream substrate mixed lineage kinase domain-like protein (MLKL) have emerged as the key cellular components in programmed necrotic cell death. Receptors for the cytokines of tumor necrosis factor (TNF) family and Toll-like receptors (TLR)
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