For continuous use, this product should be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/293/677/d9663dat-ms.pdf
D9663
Donkey serum
Synonym(s):
Serum
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Biological source:
donkey
Sterility:
non-sterile
Shipped in:
dry ice
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biological source
donkey
Quality Level
sterility
non-sterile
contains
preservative
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
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This Item | G9023 | R9133 | S3772 |
|---|---|---|---|
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| biological source donkey | biological source goat | biological source rabbit | biological source sheep |
| application(s) research pathology | application(s) research pathology | application(s) - | application(s) - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| contains preservative | contains preservative | contains preservative | contains preservative |
General description
Donkey serum is pooled from a normal donor herd. In immunoassays, normal donkey serum can serve as a blocking agent and a control. It is used as a blocking agent in various studies for immunohistochemistry and immunocytochemistry, ranging from 5% to 20%.[1]
Application
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Marianne O Larsen et al.
American journal of physiology. Endocrinology and metabolism, 282(6), E1342-E1351 (2002-05-15)
Nonrodent models of diabetes are needed for practical and physiological reasons. Induction of mild insulin-deficient diabetes was investigated in male Göttingen minipigs by use of streptozotocin (STZ) alone (75, 100, and 125 mg/kg) or 125 mg/kg combined with pretreatment with
Omid Ahmadi et al.
Journal of gastroenterology and hepatology, 25(2), 277-285 (2009-10-02)
Interstitial cells of Cajal (ICC) are distributed with smooth muscle throughout the gastrointestinal tract and are involved in regulating motility. ICC were recently discovered in the wall of the human gallbladder. This study sought to determine whether ICC are present
Yasmin Fardghassemi et al.
Disease models & mechanisms, 10(12), 1465-1480 (2017-10-25)
Polyglutamine expansion diseases are a group of hereditary neurodegenerative disorders that develop when a CAG repeat in the causative genes is unstably expanded above a certain threshold. The expansion of trinucleotide CAG repeats causes hereditary adult-onset neurodegenerative disorders, such as
H F Irving-Rodgers et al.
Molecular reproduction and development, 73(10), 1292-1302 (2006-07-26)
Using immunohistochemistry and RNA analyses we examined the fate of components of a newly identified matrix that develops between granulosa cells (focimatrix, abbreviated from focal intraepithelial matrix) and of the follicular basal lamina in ovulating bovine ovarian follicles. Pre- and
Helen F Irving-Rodgers et al.
Biology of reproduction, 69(6), 2022-2028 (2003-08-22)
Bovine ovarian antral follicles exhibit either one or the other of two patterns of granulosa cell death in atresia. Death can commence either from the antrum and progress toward the basal lamina (antral atresia) or the converse (basal atresia). In
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Related Content
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Can this product be frozen and thawed without affecting the quality?
1 answer-
Helpful?
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where can I find the protocol to reconstitute this donkey serum
1 answer-
A protocol is not associated with this product. A preservative is added to this serum, making it unsuitable for cell culture. However, in blocking applications, it is typically used at a concentration of 5% - 20%. It may be helpful to view the 'Peer Reviewed Papers' and 'Protocols and Articles' section of the product detail page.
Helpful?
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Is heat inactivation required for D9663 in immunofluorescence (IF)? I found information stating that it is not necessary for immunohistochemistry (IHC). If this applies to IF as well, what is the reason behind it?
1 answer-
Donkey serum does not require heat inactivation for immunofluorescence. It is important to note that this is not a sterile product. While the lab may have routinely heat inactivated their serums for immunofluorescence, this is likely for worker safety rather than a specific requirement for performing immunofluorescence.
Helpful?
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Does donkey serum D9663-10ML require heat inactivation prior to immunohistochemical assay?
1 answer-
There is no need to heat inactivate donkey serum for blocking purposes.
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