In undiluted form or diluted to working concentration, this material may be stored at -20°C in aliquots up to the recommended retest date for the specific lot. This data is published in the Certificate of Analysis. Avoid repeated freeze/thaw cycles. The solution may be transferred to smaller vials in working volumes for long-term storage. Plastic vials are recommended as this protein tends to adhere to glass. Please see the link below to access the product datasheet for additional details:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/686/l2020pis-ms.pdf
L2020
Mouse Laminin
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 0.2 μm filtered, liquid, 1-2 mg/mL, suitable for cell culture
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
Synonym(s):
Laminin
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Product Name
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture
biological source
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Quality Level
product line
BioReagent
form
aqueous solution
mol wt
A subunit 400 kDa
B1 subunit 210 kDa
B2 subunit 200 kDa
packaging
pkg of 1 mg
concentration
1-2 mg/mL in Tris-buffered saline
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑2 μg/cm2
impurities
Microbial Contamination, passes test
NCBI accession no.
Binding Specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)
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This Item | L6274 | C0543 | 11243217001 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane) | biological source human placenta | biological source mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane) | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
| concentration 1-2 mg/mL in Tris-buffered saline | concentration 0.5 mg/mL in Tris-buffered saline | concentration - | concentration - |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 100 |
| mol wt A subunit 400 kDa, B2 subunit 200 kDa, B1 subunit 210 kDa | mol wt - | mol wt - | mol wt 900 kDa |
| form aqueous solution | form liquid | form lyophilized powder | form solution |
General description
Laminins are non-collagenous adhesive glycoproteins[1] with a substantial molecular weight. They are heterotrimeric proteins with three disulfide-linked polypeptides known as the α, β, and γ chains.[2] The multiple polypeptide domains of laminins can bind to multiple cell surface receptors, including several integrin dimers, dystroglycan and syndecans, and other ECM components. This extracellular matrix multidomain trimeric glycoprotein is the main component of basal lamina that supports adhesion, proliferation and differentiation.
Application
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in:
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Biochem/physiol Actions
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.
Preparation Note
This product is supplied at a concentration of 1 − 2 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is filtered using a 0.2μm filter. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.
Other Notes
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product was isolated from mouse Engelbreth-Holm-Swarm tumor.
Disclaimer
It is recommended to store this product in working aliquots at -20°C.
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Mitogenic activity of laminin on human melanoma and melanocytes: different signal requirements and role of beta 1 integrins.
Mortarini R, et al.
Cancer Research, 55(20), 4702-4710 (1995)
Axonal pathfinding: Extracellular matrix role
Letourneau, P
Encyclopedia of Neuroscience, 1, 1139-1145 (2009)
Tobias Bergström et al.
Biochimica et biophysica acta, 1840(8), 2526-2532 (2014-01-28)
It is becoming increasingly apparent that the extracellular matrix acts as an important regulator of the neural stem niche. Previously we found that neural stem and progenitor cells (NSPCs) derived from the early postnatal subventricular zone of mice adhere to
Agnese Loda et al.
Nature communications, 8(1), 690-690 (2017-09-28)
Xist is indispensable for X chromosome inactivation. However, how Xist RNA directs chromosome-wide silencing and why some regions are more efficiently silenced than others remains unknown. Here, we explore the function of Xist by inducing ectopic Xist expression from multiple
Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor.
Thepparit C and Smith DR.
Journal of Virology, 78(22), 12647-12656 (2004)
Articles
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
Protocols
Các bề mặt lớp phủ với tấm laminin để nuôi cấy tế bào đòi hỏi các điều kiện cụ thể để có kết quả tối ưu. Các giao thức để lớp phủ chồng chéo với các hình cầu thần kinh nuôi cấy và nuôi cấy tế bào nói chung được bao gồm.
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
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How long can stocks be frozen at -20C for?
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How will the product L2020 shipped? Will it be shipped in room temperature, on ice, or on dry ice?
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The product L2020 will be shipped using dry ice. Upon receipt, it should be stored at -20 degrees Celsius.
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How is this laminin concentration determined? (by which method?) I tried to measure the concentration of this product by ELISA, and the results always came out half of what is written on the vial.. what is the protein purity?
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Laminin concentration is 1.0 - 2.0 mg protein/ml. The protein content is determined by Lowry method. Please refer the attached document for more details. https://www.sigmaaldrich.com/specification-sheets/131/942/L2020-1MG_________SIGMA____.pdf
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What is the protein concentration of the solution of Product L2020, Laminin?
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The protein specification for L2020 is 1.0 to 2.0 mg/mL. The exact value can be found on the lot-specific Certificate of Analysis, which can be downloaded from our website.
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Can I coat Laminin, Product L2020, on top of poly-L-lysine for better adherence?
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When coating coverslips or slides for use with neural stem cells (neurospheres), the following procedure is recommended. Precoat the coverslips with poly-L-lysine (Product No. P5899) at 100 μg/mL. This is incubated for 30 minutes or more at room temperature. The coverslips are then washed with sterile water and allowed to dry. The poly-lysine coated coverslips can be stored at room temperature until ready to use (up to ~1 month). Coverslips are then coated with 30 - 50 μL of warmed laminin, which is at a concentration of 50 μg/mL. Incubate at 37°C for 30 minutes. Remove the laminin, and wash with DME (50 μL) twice. Cells are then added to the coverslips.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I store solutions of Laminin, Product L2020?
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The product has a recommended storage temperature of -20°C. Please note, the product needs to be thawed slowly at 2-8°C. If the product has been warmed up at room temperature and it gels, it cannot be reactivated for use. We recommend storage at 2-8°C for up to one week. For longer storage, the product needs to be frozen at -20°C in single-use aliquots.
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What type of laminin is Product L2020?
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In the nomenclature system by Burgeson, this is Laminin 1. The laminins were numbered with Arabic numerals in the order discovered. The previous A, B1, and B2 chains, and their isoforms are listed by the Greek letters, for alpha, beta, and gamma, respectively, followed by an Arabic numeral to identify the isoforms. For example, the first laminin identified from the Engelbreth-Holm-Swarm tumor (EHS) was designated laminin-1 with the chain composition α-1/β-1/γ-1. The genes for these 3 chains are LAMA1, LAMB1, and LAMC1. Reference: Burgeson, R.E., et al., A new nomenclature for the laminins. Matrix Biol., 14, 209-211 (1994).
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What is the purity of Laminin, Product L2020?
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We do not determine a purity of this product. An SDS PAGE gel is run and the pattern (3 bands) is comparable to past lots.
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