Autoclaving is not recommended with glucose/dextrose according to the product information sheet for this product. However, autoclaving can be performed without the sugar, and filter sterilized glucose/dextrose can be added afterward.
Y0626
Yeast Nitrogen Base Without Amino Acids
Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements
Synonym(s):
ynb without amino acids
Sign In to View Organizational & Contract Pricing.
Select a Size
Pricing and availability is not currently available.
About This Item
UNSPSC Code:
41106212
NACRES:
NA.85
Skip To
grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
technique(s)
microbe id | utilization test: suitable
microbiological culture: suitable
pH
5.2-5.6(0.67% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts
1 of 4
This Item | Y1250 | Y1251 | 51483 |
|---|---|---|---|
| suitability nonselective for Candida spp., nonselective for Saccharomyces spp., yeasts, nonselective for Pichia spp., nonselective for Zygosaccharomyces spp. | suitability nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp. | suitability Candida spp., Pichia spp., Saccharomyces spp., Zygosaccharomyces spp., molds, yeasts | suitability fungi, selective by low pH for Candida spp., selective by low pH for Pichia spp., selective by low pH for Saccharomyces spp., selective by low pH for Zygosaccharomyces spp., selective by low pH for molds (General Media), selective by low pH for yeasts (General Media) |
| technique(s) microbe id | utilization test: suitable, microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbe id | susceptibility testing: suitable, microbe id | utilization test: suitable, microbiological culture: suitable |
| form powder | form powder | form powder | form powder |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) microbiology | application(s) clinical testing | application(s) agriculture |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.
Yeast Nitrogen Base is considered ideal for culturing wild type yeast. The presence of amino acid is known to prevent selectable marker utilization. Hence, this media is formulated without amino acids and is useful for cloning and manipulation of yeast artificial chromosome.[1]
Application
Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.
Yeast Nitrogen Base Without Amino Acids has been used:
- in synthetic complete supplement mixture (SC)-Leu media used for Y. lipolytica culturing[2] and characterization[3]
- as a component of selective liquid media for uracil auxothrophic growth in yeast[4]
- to evaluate the transformation efficiencies of S. cerevisiae strains, under different nutrient conditions[5]
Preparation Note
1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.
Other Notes
Component, amount (μg/L unless indicated)
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
related product
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Activating and elucidating metabolism of complex sugars in Yarrowia lipolytica
Ryu S, et al.
Applied and Environmental Microbiology, 82(4), 1334-1345 (2016)
T Fernandes et al.
Journal of applied microbiology, 127(3), 845-855 (2019-06-19)
The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial
Yeast transformation efficiency is enhanced by TORC 1-and eisosome-dependent signaling
Yu SC, et al.
MicrobiologyOpen, e00730-e00730 (2018)
Patrick F Suthers et al.
Metabolic engineering communications, 11, e00148-e00148 (2020-11-03)
Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial production of organic acids by microorganisms.
Peter Hayek et al.
Microbiological research, 165(3), 250-258 (2009-07-21)
Various factors are thought to be responsible for Candida albicans virulence, such as lipases, proteases and adhesins. Many of these factors are GPI-anchored cell surface proteins responsible for pathogenicity. Hwp2 is a putative GPI-anchored protein. The purpose of this study
Articles
Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
Protocols
Kỹ thuật nuôi cấy nấm men: Các hệ thống mô hình cho các nghiên cứu sinh vật nhân chuẩn với môi trường lỏng hoặc sự phát triển của mảng thạch.
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
Related Content
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Instructions
-
Why is autoclaving this not recommended?
1 answer-
Helpful?
-
-
Can this be autoclaved, or only filter sterilized?
1 answer-
It is not recommended to autoclave Product Y0626 Yeast Nitrogen Base Without Amino Acids. If preparing an bacteriological agar, then the agar should be autoclaved separately for 15 minutes only. Please follow the instructions listed on the product detail page:
1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service