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LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column

LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column application for LC-MS

Materials

related product

Product No.
Description
Pricing

C-095

Citalopram hydrobromide solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

D-044

Duloxetine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

M-145

Milnacipran hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

S-021

Sertraline hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

T-030

Trazodone hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

V-004

Venlafaxine hydrochloride solution

1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®

50813-U

Ascentis® Express C18, 2 μm UHPLC Column

2 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express C18, 10cm x 2.1mm, 2.7 μm particles (53823-U)

column

Ascentis Express C18, 10cm x 2.1mm, 2.0 μm particles (50813-U)

mobile phase

[A] 5 mM ammonium acetate in 0.1% formic acid; [B] 0.1% formic acid in acetonitrile

gradient

10% B held  for 1 min; to 55% B in 10 min

flow rate

0.4 mL/min

pressure

8280 psi (570 bar)

column temp.

35 °C

detector

MS, ESI(+), MRM, m/z 247.138/71.958,  278.168/58.025, 372.054/148.011,   442.114/155.035,  298.014/43.96, 305.968/158.873, 325.128/108.95

injection

1 μL

Description

General description

Sample preparation: Prepare a stock solution with 100 ng/mL of compounds in synthetic urine (Cerilliant, part number S-020, lot 72112). Dilute this solution with 95:5 (5mM ammonium acetate in 0.1% formic acid in water: 0.1% formic acid in acetonitrile) to give a final concentration of 50 ng/mL. Filter through a Millex LH filter. Inject. Also prepared blank in same way.

Analysis Note

Seven modern antidepressants were analyzed from urine after a dilute and inject method using reversed-phase chromatography and mass spectrometric detection.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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