C0662

6-Carboxyfluorescein

Name: 6-Carboxyfluorescein
Brand: SIGMA
Price: 153 USD
Availability: InStock

~97% purity (HPLC), powder

Synonym(s):

6-FAM, FLUOS

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50 MG

$153.00

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₂O₇

CAS Number:

3301-79-9

Molecular Weight:

376.32

Beilstein/REAXYS Number:

54341

MDL number:

MFCD00036873

UNSPSC Code:

12171500

PubChem Substance ID:

24892266

NACRES:

NA.47

Key Documents

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Product Name

6-Carboxyfluorescein, ≥96% (HPLC)

Quality Level

200

assay

≥96% (HPLC)

form

powder

technique(s)

titration: suitable

color

dark yellow to dark orange

mp

>300 °C (lit.)

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

λ_max

492 nm at 0.1 N in NaOH

fluorescence

λ_ex 492 nm; λ_em 517 nm (pH 8.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

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This Item	C5041	D0531	21877
Sigma-Aldrich C0662 6-Carboxyfluorescein	Sigma-Aldrich C5041 6-Carboxyfluorescein diacetate	Sigma-Aldrich D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride	Sigma-Aldrich 21877 5(6)-Carboxyfluorescein
technique(s) titration: suitable	technique(s) -	technique(s) -	technique(s) -
fluorescence λ_ex 492 nm; λ_em 517 nm (pH 8.0)	fluorescence -	fluorescence λ_ex 495 nm; λ_em 516 nm	fluorescence λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0
color dark yellow to dark orange	color white to yellow	color yellow to orange	color -
solubility 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange	solubility chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow	solubility methanol: 20 mg/mL, clear to slightly hazy, yellow to orange	solubility -
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -
form powder	form powder	form powder	form powder

Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.[1]

Fluorescent dye.

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.[2]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000504009

0000476098

0000338459

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