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1.09293

KOVACS′ indole reagent

for detecting microbial indole in the identification of indole-positive and indole-negative microorganisms, suitable for microbiology

Synonym(s):

4-(Dimethylamino)benzaldehyde solution, Indole reagent, Kovac′s reagent, Kovac′s reagent for indoles

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Pack SizeSKUAvailabilityPrice
100 mL
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$66.50

About This Item

NACRES:
NA.21
UNSPSC Code:
41106212

$66.50

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Quality Level

200

form

liquid

packaging

pkg of 100 mL

technique(s)

microbe id | specific enzyme detection: suitable

color

yellow

pH

<1 (20 °C in H2O)

transition temp

flash point 36 °C

density

0.92 g/cm3 at 20 °C

application(s)

microbiology

storage temp.

2-8°C

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1.113506730960983
Millipore

Millipore

1.09293

KOVACS′ indole reagent

Bactident&#8482; Kovac&#8242;s Indole reagent Available in a practical dropper bottle, for Escherichia coli

Millipore

1.11350

Bactident™ Kovac′s Indole reagent

Kovac’s reagent for indoles suitable for microbiology

Millipore

67309

Kovac′s reagent for indoles

Kovac’s reagent for indoles suitable for microbiology

Millipore

60983

Kovac′s reagent for indoles

packaging

pkg of 100 mL

packaging

pkg of (1 x 30 ml, Plastic bottle)

packaging

-

packaging

-

density

0.92 g/cm3 at 20 °C

density

0.92 g/cm3 at 20 °C

density

-

density

-

color

yellow

color

-

color

-

color

-

application(s)

microbiology

application(s)

environmental
food and beverages

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

form

liquid

form

liquid (ready-to-use)

form

-

form

-

suitability

Escherichia coli, enterovirulent E. coli (EEC) spp.

suitability

Enterobacter spp., Escherichia coli

suitability

Escherichia coli, coliforms

suitability

Escherichia coli, coliforms

General description

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Application

Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Preparation Note

No metal containers. Keep the container tightly closed in a dry and well-ventilated place. Keep away from heat and sources of ignition

Analysis Note

Indole formation (Escherichia coli ATCC 25922 (WDCM 00013)): +
Indole formation (Proteus vulgaris ATCC 8427): +
Indole formation (Enterobacter aerogenes ATCC 13048): -
Tested in DEV-Tryptophan broth art. no. 1.10694. (after incubation 24 hrs.; 35 °C aerobic).

Other Notes

The reagent solution must be stored in the dark in the refrigerator, otherwise it may turn brown and cannot be used.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

96.8 °F

flash_point_c

36 °C

Certificates of Analysis (COA)

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Protocols

Salmonella Detection in the Food Chain (EN ISO 6579-1)

EN ISO 6579-1 standard specifies detection of Salmonella spp. in human consumption, animal feed, and environmental samples.

Global Trade Item Number

SKUGTIN
109293010004022536113227

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