GF174
Sonic HedgeHog (Shh) Protein, Human Recombinant
The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene.
Synonym(s):
SHH, HHG-1
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| Size/SKU | Availability | Price |
|---|---|---|
25 μg | Check Cart for Availability | $430.00 |
About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Assay:
>98% (SDS-PAGE and HPLC)
$430.00
Check Cart for Availability
General description
Product Source: E. coli.
The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoietic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Cross Reactivty
None
None
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| assay >98% (SDS-PAGE and HPLC) | assay ≥98% (HPLC), ≥98% (SDS-PAGE) | assay >98% (SDS-PAGE and HPLC) | assay >98% (SDS-PAGE and HPLC) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C |
| impurities <0.1 μg/mg protein endotoxin (<1EU/μg) | impurities <0.1 EU/μg endotoxin, tested | impurities <0.1 μg/mg protein endotoxin (<1EU/μg) | impurities <0.1 μg/mg protein endotoxin (<1 EU/μg) |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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