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RPOLT3-RO

Roche

T3 RNA Polymerase

Name: T3 RNA Polymerase
Brand: ROCHE
Price: 122.32 CAD

from Escherichia coli HB101

Synonyme(s) :

mRNA, polymerase

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À vous/Référence	Disponibilité	Prix
1000 units	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	139,00 $ 122,32 $

A propos de cet article

UNSPSC Code:

12352204

Numéro CE :

2.7.7.6 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

≥20 U/μL

Assay:

100% (SDS-PAGE)

Biological source:

Escherichia coli (HB101)

Concentration:

<0.1 % (w/w)

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Propriétés

biological source

Escherichia coli (HB101)

assay

100% (SDS-PAGE)

form

solution

specific activity

≥20 U/μL

mol wt

100 kDa (single polypeptide chain)

packaging

pkg of 1,000 U (11031163001), pkg of 5,000 U (11031171001)

manufacturer/tradename

Roche

concentration

<0.1 % (w/w)

technique(s)

DNA sequencing: suitable, Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable

color

colorless

pH

7.9 (39 °F), 8.0 (68 °F)

solubility

water: miscible

suitability

suitable for molecular biology

NCBI accession no.

NP_418414

UniProt accession no.

P0A8V2

application(s)

genomic analysis
life science and biopharma

foreign activity

Nicking activity 150 units, none detected, RNases 150 units, endonucleases 150 units, none detected

storage temp.

−20°C

Gene Information

Escherichia coli ... rpoB(948488)

Description

General description

With this enzyme, homogeneously labeled single-stranded RNA can be generated. Transcripts can be nonradioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-³²P]- or [α-³⁵S]-labeled nucleotides. T3 RNA polymerase is a DNA-dependent RNA polymerase that is highly specific for its promoter. Only DNA downstream from the T3 promoter will be transcribed.

Application

T3 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from a T3 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

RNA or DNA blotting techniques
In situ hybridization
RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.

Biochem/physiol Actions

Promotor specifity
T3 RNA polymerase is extremely promoter-specific and only transcribes bacteriophage T3 DNA or DNA cloned downstream of a T3 promoter.
Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or heat to 65 °C.

Features and Benefits

Volume Activity: =20U/μl
Synthesis of hybridization probes: T3 RNA polymerase allows highly efficient production of homogeneously labeled RNA. This labeled RNA may be used as hybridization probes in Southern, northern, and dot blots, as well as in situ hybridizations.
Suitable labels: Transcripts can be nonradioactively labeled with biotin-16-UTP, DIG-11-UTP, or fluorescein-12-UTP. They may also be radioactively labeled to high specific activity with [a-32P]- or [a-35S]-labeled nucleotides.

Note: Roche has 10x concentrated RNA Labeling Mixes that are specially designed for DIG-, biotin-, and fluorescein-labeling. These mixes work well with T3 RNA Polymerase.

Packaging

1 kit containing 2 components

Preparation Note

Activator: BSA/spermine

Keep container tightly closed in a dry and well-ventilated place.

Analysis Note

Absence of endonucleases, nicking activity, and RNases tested according to the current Quality Control procedures. Function tested with the SP6/T7 Transcription Kit.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity which incorporates 1 nMol CMP in acid-precipitable RNA products within 60 minutes at +37 °C.

Volume Activity: ≥20 U/μl

Comparer avec des articles similaires

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Montrer les différences

1 of 1

Cet article	RPOLT7-RO	RPOLSP6-RO	11480022001
Roche RPOLT3-RO T3 RNA Polymerase	Roche RPOLT7-RO T7 RNA Polymerase	Roche RPOLSP6-RO ARN polymérase SP6	Roche 11480022001 Tth DNA Polymerase
Gene Information Escherichia coli ... rpoB(948488)	Gene Information Escherichia coli ... T7p07(1261050)	Gene Information Escherichia coli ... rpoB(948488)	Gene Information -
specific activity ≥20 U/μL	specific activity ≥20 U/μL	specific activity ≥20 U/μL	specific activity 5 U/μL
biological source Escherichia coli (HB101)	biological source Escherichia coli (BL 21/pAR 1219)	biological source Escherichia coli (BL 21/pSR3)	biological source bacterial (Thermus thermophilus)
assay 100% (SDS-PAGE)	assay 100% (SDS-PAGE)	assay 100% (SDS-PAGE)	assay -
technique(s) DNA sequencing: suitable, Southern blotting: suitable, Northern blotting: suitable, hybridization: suitable	technique(s) Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable	technique(s) DNA sequencing: suitable, Northern blotting: suitable, Southern blotting: suitable	technique(s) PCR: suitable, RT-PCR: suitable, nucleic acid labeling: suitable
form solution	form solution	form solution	form liquid

Composants de kit seuls

Réf. du produit

Description

T3 RNA Polymerase, in buffer, pH 7.9 ≥20 U/μl

Transcription Buffer 10x concentrated

pictograms

GHS07

signalword

Warning

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

does not flash

flash_point_c