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o-Phenylenediamine dihydrochloride

Name: <I>o</I>-Phenylenediamine dihydrochloride
Brand: SIGMA
Price: 47400 JPY

chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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Size/SKU	Availability	Price
50 tabs	Check Cart for Availability	¥47,400
100 tabs	Check Cart for Availability	¥81,700

About This Item

Linear Formula:

C₆H₄(NH₂)₂·2HCl

CAS Number:

615-28-1

Molecular Weight:

181.06

NACRES:

NA.83

PubChem Substance ID:

24898919

UNSPSC Code:

12352204

MDL number:

MFCD00012966

Beilstein/REAXYS Number:

3912045

¥47,400

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Properties

Product Name

o-Phenylenediamine dihydrochloride, tablet, 30 mg substrate per tablet

Quality Segment

200

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

o-Phenylenediamine dihydrochloride is a binucleophile and its condensation with α,β-unsaturated carbonyl compounds and aldehydes yields benzimidazoles.

Application

o-Phenylenediamine dihydrochloride has been used as a substrate for horse radish peroxidase in detecting:

human immunoglobulin
CTLA4Ig in serum samples
albumin in culture medium

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

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This Item	P4664	P3804	P6912
Sigma-Aldrich P8412 o-Phenylenediamine dihydrochloride	Sigma-Aldrich P4664 o-Phenylenediamine dihydrochloride	Sigma-Aldrich P3804 o-Phenylenediamine dihydrochloride	Sigma-Aldrich P6912 o-Phenylenediamine dihydrochloride
description tablet, 30 mg substrate per tablet	description tablet, 15 mg substrate per tablet	description tablet, 5 mg substrate per tablet	description tablet, 5 mg substrate per tablet
solubility water: 1 tablet/10 mL, clear, colorless	solubility water: 1 tablet/10 mL, clear, colorless	solubility water: 1 tablet/10 mL, clear, colorless	solubility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 300
form tablet	form tablet	form tablet	form tablet
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
mp 258 °C (dec.) (lit.)	mp 258 °C (dec.) (lit.)	mp 258 °C (dec.) (lit.)	mp 258 °C (dec.) (lit.)

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pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302 + H332,H317,H319,H341,H351,H410

pcodes

P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

P8412-50TAB:4.548173398334E12 + P8412-100TAB:4.548173398327E12

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