1.00432
ChromoCult® Listeria Agar Selective-Supplement
for Listeria spp., pkg of 10 vials, for the preparation of ChromoCult® Listeria agar (base)
Synonym(s):
Listeria Selective Supplement
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106212
Product 1.00432 is not currently sold in your country. Contact Technical Service
agency
ISO 11290
Quality Segment
product line
ChromoCult®
form
lyophilized powder
composition
Amphotericin B, 5 mg per vial , Ceftazidime, 10 mg per vial , Nalidixic acid sodium salt, 10 mg per vial , Polymyxin B sulphate, 38350 (U per vial)
packaging
pkg of 10 vials
manufacturer/tradename
ChromoCult®
technique(s)
microbiological culture: suitable
pH
6.2 (20 °C in H2O, Aqueous solution)
application(s)
food and beverages
microbiology
compatibility
for use with ChromoCult® Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)
storage temp.
2-8°C
suitability
Listeria spp.
General description
ChromoCult® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.
Application
FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult® Listeria agar (base) (1.00427) for the selective growth of Listeria spp.
Analysis Note
Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.
Other Notes
Replaces: 1.00432.0017
Legal Information
CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Carc. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
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