The presence of DTT in 560000 may be inhibiting signal detection by quenching the fluorescence reading from PCR. It is advisable to remove DTT before conducting PCR analysis.
560000
Sputolysin® Reagent
Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.
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| Size/SKU | Availability | Price |
|---|---|---|
10 mL | Check Cart for Availability | $96.00 $72.00 |
1 set | Check Cart for Availability | $600.00 |
About This Item
Form:
liquid
Quality Segment
description
RTECS - EK1610000
form
liquid
reaction suitability
reagent type: reductant
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
shipped in
ambient
storage temp.
15-25°C
SMILES string
SC[C@H](O)[C@@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChI key
VHJLVAABSRFDPM-IMJSIDKUSA-N
General description
A concentrate of dithiothreitol (DTT) in phosphate buffer. Useful for the isolation of pathogenic or saprophytic bacteria, fungi, yeast, and epithelial cells from sputum and other mucoid fluids.
Concentrate of Dithiothreitol (DTT; Cat. No. 233155) in phosphate buffer, pH 6.5-7.5. Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. Each vial is sufficient to make 100 ml of working solution.
Note: 1 set = 20 x 10 ml.
Physical form
20 vials each containing 10 ml of concentrated DTT OR 1 vial containing 10 ml of concentrated DTT.
Preparation Note
Dilute the contents of one vial to 100 ml with sterile distilled water. Swirl gently to dissolve any crystals. If the diluted material appears cloudy, the solution may be filtered to remove any insoluble particles. The diluted solution is ~6.5 mM dithiothreitol in 100 mM phosphate buffer, pH 7.0.
Once a vial is opened the unused concentrate should be purged with an inert gas (e.g. N₂) and refrigerated (4°C). Diluted solutions, stored aseptically under an inert gas, are stable for up to 48 h at 4°C.
Other Notes
Hirsch, S.R., et al. 1969. J. Lab. Clin. Med.74, 346.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.
Shah, R.R. and Dye, W.E. 1966. Am. Rev. Respir. Dis.94, 454.
Dixon, J.M.S. and Miller, D.C. 1965 (November). Lancet p. 1046.
Cleland, W.W. 1964. Biochemistry3, 480.
May, J.R. 1953 (September). Lancet p. 535.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
SPUTOLYSIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
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This Item | |||
|---|---|---|---|
| description Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum. | description A protective agent for SH groups. | description Molecular Biology, ≥98% (HPLC), ≥99% (titration) | description BioUltra, Molecular Biology, ≥99.5% (RT) |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| reaction suitability reagent type: reductant | reaction suitability reagent type: reductant | reaction suitability reagent type: reductant | reaction suitability reagent type: reductant |
| storage temp. 15-25°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| form liquid | form powder, solid | form powder | form powder |
| storage condition protect from light, OK to freeze | storage condition OK to freeze | storage condition - | storage condition - |
| description RTECS - EK1610000 | description Merck USA index - 14, 3376 | description - | description - |
signalword
Warning
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
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Is there any significant interference in the signal after using 560000 for sample preparation and PCR analysis?
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