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ACK5010PG

Amicon® Pro Affinity Concentration Kit Protein G with 10kDa Amicon® Ultra-0.5 Device

Amicon Pro Affinity Concentration Kit Protein G - A Centrifugal Tool for purifying & concentrating Antibodies.

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About This Item

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
Pore size:
10 kDa NMWCO
Working volume:
10 mL
Sterility:
non-sterile
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material

Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)

sterility

non-sterile

product line

Amicon®

manufacturer/tradename

Amicon® Pro

parameter

10 mL sample volume

technique(s)

buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)

L

12 cm (4.7 in.)

diam.

2.97 cm

filtration area

1 cm2

working volume

10 mL

pore size

10 kDa NMWCO

shipped in

wet ice

General description

Filter Code: PLCGC
Filter Type: Ultrafiltration
This kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 10 kDa, 3 ml Protein G resin (Binding capacity: 20mg human IgG/ml agarose), 2 X 66ml Bind/Wash Buffer, 12 ml Elution buffer, 2.5 ml Neutralization buffer

Application

Amicon Pro Affinity Concentration Kit Protein G - A Centrifugal Tool for purifying & concentrating Antibodies.

Features and Benefits

Minimum Final Concentrate Volume: 15 µL

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

FlameCorrosion
GHS02,GHS05

signalword

Danger

hcodes

H225,H290

Hazard Classifications

Flam. Liq. 2 - Met. Corr. 1

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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bios-C-epdf-1043-1304-SP

Amicon® Pro Affinity Concentration Kit - Protein G
Simultaneous cell lysis and capture using the Amicon®Pro device

E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.

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Global Trade Item Number

SKUGTIN
ACK5010PG-B04055977151107