DIGUTP-RO

Roche

Digoxigenin-11-UTP

Name: Digoxigenin-11-UTP
Brand: ROCHE

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About This Item

NACRES:

NA.21

UNSPSC Code:

41105500

Assay:

≥85% (HPLC)

Form:

solution

Storage temp.:

−20°C

Key Documents

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assay

≥85% (HPLC)

form

solution

mol wt

(M_r= 1106.7 (DIG-11-UTP-Li4))

packaging

pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

Labware Partners

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Labeling

Roche Life Science Products

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Show Differences

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This Item	11363905910	DIUTP-RO	DIUTPS-RO
Roche DIGUTP-RO Digoxigenin-11-UTP	Roche 11363905910 Digoxigenin-11-ddUTP	Roche DIUTP-RO Digoxigenin-11-dUTP, alkali-labile	Roche DIUTPS-RO Digoxigenin-11-dUTP, alkali-stable
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
form solution	form solution	form solution	form solution
assay ≥85% (HPLC)	assay 85% (HPLC)	assay 85% (HPLC)	assay ≥85% (HPLC)
packaging pkg of 25 μL (11209256910 [10 mM]), pkg of 57 μL (03359247910 [3.5 mM])	packaging pkg of 25 μL (25 nmol; 1mM)	packaging pkg of 125 μL (11573179910 [1 mM]]]), pkg of 25 μL (11573152910 [1 mM])	packaging pkg of 125 μL (11558706910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]), pkg of 25 μL (11093088910 [1 mM])
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases.[1][2] It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:

in situ hybridization[3][4]
in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
fluorescence in situ hybridization (FISH)

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Analysis Note

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C₄₃H₆₁N₄O₂₂P₃Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 + P330 - P501

Hazard Classifications

Acute Tox. 4 Oral

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

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Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide

<BIG>Kalra A and Xia D</BIG>

Arsenal (2017)

In situ hybridization shows Dmp1 (AG1) to be a developmentally regulated dentin-specific protein produced by mature odontoblasts.

A George et al.

Connective tissue research, 33(1-3), 67-72 (1995-01-01)

Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from

Whole mount RNA fluorescent in situ hybridization of Drosophila embryos.

Félix Legendre et al.

Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)

Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing

Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution

Biotechniques, 56 (2014)

Isolation and functional characterization of two dioxygenases putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)

<BIG>Carballo U, et al.</BIG>

PeerJ, 7 (2019)

View All Related Papers

Global Trade Item Number

SKU	GTIN
3359247910	04061826742723
11209256910	04061837902062

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