DIGUTP-RO

Digoxigenin-11-UTP

• UNSPSC Code: 41105500
• NACRES: NA.21

Properties

• assay: ≥85% (HPLC)
• Quality Level: 100
• form: solution
• mol wt: (M_r = 1106.7 (DIG-11-UTP-Li4))
• packaging: pkg of 25 μL (11209256910 [10 mM]); pkg of 57 μL (03359247910 [3.5 mM])
• manufacturer/tradename: Roche
• storage temp.: −20°C

Description

General description

Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases.[1][2] It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.

Application

Digoxigenin-11-UTP has been used for labeling RNA probes in:

• in situ hybridization[3][4][5]
• in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)[6]
• fluorescence in situ hybridization (FISH)[7]

Biochem/physiol Actions

Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.

Quality

Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.

Formula: C₄₃H₆₁N₄O₂₂P₃Li₄

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 + P330 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash