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Fibronectin (pure)

Name: Fibronectin (pure)
Brand: ROCHE

from human plasma, >95% (SDS-PAGE), lyophilized (clear, colorless solution after reconstitution)

Synonym(s):

Fibronectin

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About This Item

UNSPSC Code:

12352202

MDL number:

MFCD00131062

NACRES:

NA.54

Biological source:

human plasma

Form:

lyophilized (clear, colorless solution after reconstitution)

Technique(s):

cell culture | mammalian: suitable

Mol wt:

440 kDa

Assay:

>95% (SDS-PAGE)

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Properties

biological source

human plasma

sterility

non-sterile; 0.2 μm filtered

assay

>95% (SDS-PAGE)

form

lyophilized (clear, colorless solution after reconstitution)

mol wt

440 kDa

packaging

pkg of 1 mg (11051407001), pkg of 5 mg (11080938001)

manufacturer/tradename

Roche

technique(s)

cell culture | mammalian: suitable

UniProt accession no.

P02751

storage temp.

2-8°C

Gene Information

human ... FN1(2335)

Description

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.[1]

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes.[2] It has been used for the cell adhesion assay.[3][4]

Biochem/physiol Actions

Active on most mammalian cells
Biological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Preparation Note

Working concentration: 5μg/cm² for the coating of cell culture vessels
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.
Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Show Differences

1 of 1

This Item	10838039001	F2733	MAB88916-C
Roche FIBRP-RO Fibronectin (pure)	Roche 10838039001 Fibronectin	Sigma-Aldrich F2733 Fibronectin-FITC	Sigma-Aldrich MAB88916-C Anti-Fibronectin (Cell Attachment Fragment) Antibody, clone 3E3, Ascites & Azide Free
sterility non-sterile; 0.2 μm filtered	sterility non-sterile; 0.2 μm filtered	sterility -	sterility -
biological source human plasma	biological source human plasma	biological source human	biological source mouse
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, inhibition assay: suitable, western blot: suitable
assay >95% (SDS-PAGE)	assay 95% (SDS-PAGE)	assay -	assay -
form lyophilized (clear, colorless solution after reconstitution)	form lyophilized (clear, colorless solution after reconstitution)	form liquid	form -
mol wt 440 kDa	mol wt 440 kDa	mol wt -	mol wt -

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Protocole de coating de fibronectine

Diluer la fibronectine à la concentration souhaitée. Les conditions d'adhésion optimales dépendent du type de cellules et de l'application. La concentration de coating standard varie de 1 à 5 µg/cm². Protocole de coating de fibronectine, produits et FAQ.

Global Trade Item Number

SKU	GTIN
11080938001	04061838698292
11051407001	04061838697493