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KAPA LongRange

Name: KAPA LongRange
Brand: ROCHE
Price: 341 EUR

HotStart PCR Kit

Synonym(s):

PCR

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Pack Size	SKU	Availability	Price
250 U		Please contact Customer Service for Availability	€341.00
500 U		Please contact Customer Service for Availability	€634.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

€341.00

Please contact Customer Service for Availability

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Properties

usage

sufficient for 150 reactions, sufficient for 400 reactions, sufficient for 800 reactions

shelf life

≤18 mo.

feature

High Fidelity PCR, Long & Accurate PCR, dNTPs included, hotstart

packaging

pkg of 100 U (KK3501), pkg of 250 U (KK3502), pkg of 500 U (KK3503)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

Description

General description

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long-range and/or sensitive PCR, and possess 3 – 4X higher fidelity than Taq polymerase. In the hot-start formulation, KAPA Long Range is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity.

KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template. The loading dye in the reaction buffer allows you to load PCR products directly onto the agarose gel with no extra steps.

Application

KAPA LongRange has been used in:

Amplification of long targets and/or low amounts
Standard short- and mid-range PCR
T-A cloning
Targeted sample enrichment for next-generation DNA sequencing
polymerase chain reaction (PCR)[1][2]

Biochem/physiol Actions

Both KAPA Taq HotStart^® DNA polymerase and a modified archaeal (Type B) DNA polymerase enzyme possess 5′→3′ polymerase activity, but only KAPA Taq HotStart DNA polymerase possesses 5′→3′ exonuclease activity and only the Type B DNA polymerase possesses 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA LongRange HotStart is 2–4 fold higher than that of Taq DNA Polymerase, but is lower than the fidelity of pure proofreading polymerases such as KAPA HiFi. Reaction products generated with KAPA LongRange HotStart are polyA-tailed and can be cloned into TA-cloning vectors.

Features and Benefits

Amplify long targets with high sensitivity:

Amplification of targets with high sensitivity and specificity up to 15 kb
High yields with low input DNA

Outperforms with complex targets:

Exhibits high yield, greater sensitivity and specificity where competitor long-range systems fail

Quick Notes:

KAPA LongRange HotStart is a blend of KAPA Taq HotStart and a proofreading DNA polymerase.
Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA.
Fidelity is 2–4 times better than Taq.For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity.
Amplify mid- to long-range targets with high yield and sensitivity.
KAPA LongRange Buffer (Mg-free) is designed for long-range, sensitive PCR. MgCl₂ issupplied separately for optimization.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for longterm storage.

Analysis Note

Each batch of KAPA LongRange HotStart DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). The KAPA LongRange HotStart PCR system is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

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This Item	LRHSRMKB	SFLRAB	2GFRMKB
Roche LRHSNTKB KAPA LongRange	Roche LRHSRMKB KAPA LongRange	Roche SFLRAB KAPA SYBR^® FAST	Roche 2GFRMKB KAPA2G Fast ReadyMix
technique(s) PCR: suitable	technique(s) PCR: suitable	technique(s) qPCR: suitable	technique(s) PCR: suitable
feature High Fidelity PCR, dNTPs included, Long & Accurate PCR, hotstart	feature Long Range PCR, dNTPs included, hotstart	feature dNTPs included, hotstart	feature Fast PCR, dNTPs included, hotstart: no
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
input purified DNA	input purified DNA	input purified DNA	input purified DNA
usage sufficient for 150 reactions, sufficient for 800 reactions, sufficient for 400 reactions	usage sufficient for 100 reactions, sufficient for 500 reactions	usage sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions, sufficient for 5000 reactions	usage sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche

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Kit Components Only

Product No.

Description

KAPA Long Range HotStart^® DNA Polymerase 5 U/μL or 2.5 U/μL

5X KAPA Long Range Buffer

MgCl₂ 25 mM

dNTP Mix 10 mM each dNTP

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Articles

Directed Evolution

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Global Trade Item Number

SKU	GTIN
KK3502	04061834429500
KK3501	04061834429494
KK3503	04061834429517