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RDLIG-RO

Roche

Rapid DNA Dephos & Ligation Kit

sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C

Synonym(s):

cloning of dna fragments, dna ligase, ligases

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40 reactions
0 left in stock
$257.00
160 reactions
0 left in stock
$685.00

About This Item

EC Number:
3.1.3.1 (BRENDA, IUBMB)
NACRES:
NA.55
UNSPSC Code:
12352200

$257.00

0 left in stock
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usage

sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001)

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

storage temp.

−20°C

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This Item
11635379001LIG2DNALIG-RO
Rapid DNA Dephos & Ligation Kit sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C

Roche

RDLIG-RO

Rapid DNA Dephos & Ligation Kit

Rapid DNA Ligation Kit

Roche

11635379001

Rapid DNA Ligation Kit

QuickLink™ DNA Ligation Kit joins blunt-end and sticky-end DNA fragments

Sigma-Aldrich

LIG2

QuickLink™ DNA Ligation Kit

T4 DNA Ligase solution, from Escherichia coli(NM 989), suitable for activity assay

Roche

DNALIG-RO

T4 DNA Ligase

usage

sufficient for 160 reactions (04898125001), sufficient for 40 reactions (04898117001)

usage

sufficient for 40 reactions (DNA ligation)

usage

1 kit sufficient for 50 ligation reactions

usage

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

parameter

37 °C optimum reaction temp.

parameter

-

parameter

-

parameter

4-22 °C optimum reaction temp.

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

General description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.

Application

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
  • Cloning of fragments into either plasmid or phage vectors[1]
  • Linker ligation
  • Recircularization of linear vector DNA
  • Generation of libraries

Biochem/physiol Actions

Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.

Packaging

1 kit containing 5 components

Other Notes

For life science research only. Not for use in diagnostic procedures.
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.

Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl

Kit Components Only

Product No.
Description
  • rAPid Alkaline Phosphatase Buffer 10x concentrated
  • rAPid Alkaline Phosphatase 1 U/μl
  • DNA Dilution Buffer 5x concentrated
  • T4 DNA Ligation Buffer 2x concentrated
  • T4 DNA Ligase 5 U/μl

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
52250800
50235900
49909400
42989000
39466000

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Francesca Colombo et al.
Molecular cancer, 10, 81-81 (2011-07-09)
The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences in allelic frequencies of the functional genetic variations.

Global Trade Item Number

SKUGTIN
489812500104061832401775
489811700104061832401768

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