Bromocresol Purple is intended for use in aqueous solutions - not for alcoholic solutions. Between pH 5.2 and 6.8, the color transition should be yellow to purple. Alcoholic solutions will need to be diluted with water before performing a titration
114375
Bromocresol Purple
BioReagent, suitable for indicator, Dye content 90 %
Synonym(s):
5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
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| Size/SKU | Availability | Price |
|---|---|---|
5 g | Available to ship TODAY | $77.10 |
25 g | Available to ship TODAY | $229.00 |
About This Item
Empirical Formula (Hill Notation):
C21H16Br2O5S
CAS Number:
Molecular Weight:
540.22
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
204-087-8
Beilstein/REAXYS Number:
359618
MDL number:
$77.10
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product line
BioReagent
Quality Segment
assay
≥89.0% (HPLC)
form
powder
composition
Dye content, 90%
technique(s)
titration: suitable
pH
5.2-6.8, yellow to purple
visual transition interval
5.2-6.8, yellow to blue
mp
240 °C (dec.) (lit.)
solubility
ethanol: 1 mg/mL
λmax
419 nm
ε (extinction coefficient)
≥22000 at 418-424 nm in methanol
suitability
suitable for indicator
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
InChI
1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
InChI key
ABIUHPWEYMSGSR-UHFFFAOYSA-N
Application
Spectrophotometric determination of serum-albumin
Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Biochem/physiol Actions
Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
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This Item | |||
|---|---|---|---|
| description BioReagent, suitable for indicator, Dye content 90 % | description indicator grade, Dye content 90 % | description - | description indicator grade, Dye content 95 % |
| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) titration: suitable | technique(s) titration: suitable |
| suitability suitable for indicator | suitability - | suitability suitable for microscopy | suitability - |
| solubility ethanol: 1 mg/mL | solubility - | solubility methanol: water (1:1): 0.1 g/10 mL, red | solubility ethanol: 1 mg/mL, clear |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder or crystals |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
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What is the difference between 0.04%w/v bromocresol in aq.solution and methylated spirit in aspects of titration volumetric quantitative analysis
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