Cycloheximide is commonly used to block protein synthesis in eukaryotic cells and in ribosome profiling experiments. It is recommended to refer to literature for further information, for example: https://pmc.ncbi.nlm.nih.gov/articles/PMC2831214/ and https://www.nature.com/articles/s41467-021-25411-y.
18079
Cycloheximide solution
0.1%, suitable for microbiology
Synonyme(s) :
CHX
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A propos de cet article
Formule empirique (notation de Hill) :
C15H23NO4
Numéro CAS:
Poids moléculaire :
281.35
NACRES:
NA.85
PubChem Substance ID:
UNSPSC Code:
41171614
MDL number:
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Service technique
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Laissez-nous vous aidersterility
sterile (Filtered and Aseptic Handled)
Quality Segment
form
liquid
shelf life
limited shelf life, expiry date on the label
concentration
0.1%
antibiotic activity spectrum
fungi
application(s)
environmental
food and beverages, microbiology
storage temp.
2-8°C
suitability
bacteria
SMILES string
O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
InChI
1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
InChI key
YPHMISFOHDHNIV-FSZOTQKASA-N
Gene Information
human ... FKBP1A(2280), PIN1(5300)
General description
Cycloheximide solution is a selective supplement, that is safer and easier to use than cycloheximide powder. The required volume can be simply added to the medium before or after sterilization.
Application
Cycloheximide solution has been used as a supplement in De Man, Rogosa and Sharpe (MRS) and GM-17 agar plates to study its effects on lactic acid bacteria (LAB).
Biochem/physiol Actions
Cycloheximide solution is used to block the protein synthesis for suppressing the growth of fungi.
Physical form
Sterile filtered solution containing 0.1% cycloheximide
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Cet article | |||
|---|---|---|---|
| description 0.1%, suitable for microbiology | description Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture | description from microbial, ≥94% (TLC) | description ≥95% (HPLC) |
| application(s) environmental | application(s) - | application(s) - | application(s) - |
| form liquid | form ready-to-use solution | form powder | form powder or crystals |
| suitability bacteria | suitability - | suitability - | suitability - |
| sterility sterile (Filtered and Aseptic Handled) | sterility - | sterility - | sterility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| shelf life limited shelf life, expiry date on the label | shelf life - | shelf life - | shelf life - |
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Can be used for ribosomal profiling, as a blocker of protein synthesis in eukaryotic cells?
1 answer-
Helpful?
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I am looking to add this solution to a Lee's Multi Differential Agar (LMDS) media solution to prevent saccharomyces cerevisiae growth on my plates. How much of this cycloheximide solution should be added per 100 ml of LMDA agar media?
1 answer-
According to brewing industry experts, cycloheximide can be added to Lee's Multi Differential Agar in order to make the media selective to bacteria and wild yeast. With cycloheximide, one can suppress the growth of most brewing yeasts without interfering with bacteria and wild yeast growth. Concentrations recommended for this purpose are in the range from 7-10 mg/L. Adding 10 mLs of product 18079 (at a concentration of 0.1%) to 1 L of agar will result in a concentration of 10 mg/L (a 1:100 dilution).
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