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73816

BG11 Broth

Name: BG11 Broth
Brand: SIAL

NutriSelect^® Basic, 100 × (Concentrate), liquid, sterile (Filtered and Aseptic Handled), for cyanobacteria, bottle of 250 mL, suitable for microbiology

Synonym(s):

Cyanobacteria BG-11 Broth, Blue green Medium

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About This Item

NACRES:

NA.74

UNSPSC Code:

41171607

eCl@ss:

32160702

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Properties

Quality Segment

100

sterility

sterile (Filtered and Aseptic Handled)

product line

NutriSelect^® Basic

form

liquid

shelf life

limited shelf life, expiry date on the label

composition

calcium chloride dihydrate, 36.7 mg/L , citric acid, 5.6 mg/L , dipotassium hydrogen phosphate, 31.4 mg/L , disodium magnesium ETDA, 1 mg/L , ferric ammonium citrate, 6 mg/L , magnesium sulfate, 36 mg/L , sodium carbonate, 20 mg/L , sodium nitrate, 1.5 g/L

packaging

bottle of 250 mL

manufacturer/tradename

NutriSelect^® Basic

concentration

100 × (Concentrate)

technique(s)

microbiological culture: suitable

final pH

4.0-4.6 (25 °C, not adjusted)

application(s)

agriculture
environmental
food and beverages
life science and biopharma, microbiology

storage temp.

2-8°C

suitability

cyanobacteria

Description

General description

BG11-Broth is a universal medium designed for the culture and maintenance of cyanobacteria. The medium is formulated with sodium nitrate as the primary nitrogen source, while dipotassium hydrogen phosphate provides essential phosphate. Magnesium sulphate and calcium chloride dihydrate supply essential mineral salts, and sodium carbonate maintains the alkaline environment required for cyanobacterial growth. Disodium magnesium EDTA acts as a chelating agent, citric acid serves as an organic acid supplement, and ferric ammonium citrate provides the iron necessary for photosynthetic processes. Cyanobacteria cultured in this medium require indirect light, as direct sunlight can be detrimental; exposure to horticultural neon lighting over 24 hours is recommended. The cultures proliferate well at normal room temperatures of approximately 20°C.

Application

BG-11 is an universal medium for the cultivation and maintenance of blue green algae (cyanobacteria), such as Synechocystis 6803; Nostoc sp. PCC 9104 and Nostoc sp. PCC 9025. It is used for studies of the photosystems, as well as to identify the primary targets of the photoprocess.

Features and Benefits

NutriSelect^® Basic is a powdered culture media offering

Quality control with growth promotion test according to industry standards
Quality assurance acc. to ISO 9001
Cost-efficient powdered media

Preparation Note

Dissolve the medium concentrate 1:100 with sterile water. It is recommended to adjust pH, under aseptic conditions, with 1M NaOH to 7.1 or as desired. Add the choosen sterile trace element solution.

Other Notes

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

GHS03,GHS07

Signal Word

Warning

Hazard Codes

H272,H319

Precautionary Statements

P210 - P220 - P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2 - Ox. Liq. 3

Storage Class

5.1B - Oxidizing hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)