After the discontinous density gradient is prepared using Histopaque 10771 and Histopaque 1119, the tubes are centrifuged for 30 minutes at 700G. The Product Information Sheet is available from the website and includes the complete recommended procedure.
11191
Histopaque®-1119
sterile-filtered, density: 1.119
Histopaque®-1119
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Sobre este item
NACRES:
NA.75
UNSPSC Code:
12352207
Preço e disponibilidade não estão disponíveis no momento.
Assistência Técnica
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Estamos aqui para ajudarsterility
sterile-filtered
Quality Segment
form
liquid
shelf life
Expiry date on the label.
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
density
1.119
application(s)
hematology
histology
storage temp.
2-8°C
General description
Histopaque®-1119 é um meio estéril testado quanto à presença de endotoxinas e pronto para uso. Esta solução consiste em polissacarose e diatrizoato de sódio, com densidade relativa ajustada para 1,119 g/ml e pH 8,8–9,0.
Application
O Histopaque®-1119 é adequado para uso em conjunto com o Histopaque-1077, pois facilita a recuperação rápida de células mononucleares e granulócitos viáveis a partir de pequenos volumes de sangue total ou medula óssea.
Biochem/physiol Actions
O processo envolve a formação de um gradiente duplo em sobreposição de um volume igual de Histopaque-1077 sobre Histopaque-1119. O sangue total anticoagulado é cuidadosamente colocado sobre o meio Histopaque-1077 superior. Após a centrifugação, os eritrócitos são agregados por polissacarose e, assim, rapidamente sedimentados. Os granulócitos são encontrados na interface inferior de Histopaque-1077/1119; enquanto os linfócitos e outras células mononucleares são encontrados na interface plasma/Histopaque-1077 superior. A maioria das plaquetas exteriores encontradas na interface superior é removida por centrifugação de baixa velocidade durante as etapas de lavagem. A contaminação por eritrócitos é insignificante.
Features and Benefits
Histopaque®-1119 oferece inúmeras vantagens, como:
- Recuperação ideal de células viáveis
- Permite a separação seletiva de tipos de células sanguíneas
- Previne a distorção celular
- Preserva a viabilidade celular
- Mínima interferência de células estranhas
- Desempenho reprodutível de lote para lote
- Filtro estéril e testado quanto a endotoxinas
- Estável a 2-8 °C por pelo menos 2 anos quando protegido da luz
Legal Information
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Este Item | |||
|---|---|---|---|
| density 1.119 | density 1.077 | density 1.083 | density 1.077 g/mL |
| form liquid | form liquid | form liquid | form solution |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. |
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Classe de armazenamento
10 - Combustible liquids
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Bei wieviel g muss das geschichtete (1,1077/1,119/Blut) und wie lange zentrufugiert werden um eine Trennung zu erreichen?
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Is the Histopaque-1119 solution compatible with blood stored in PAXgene cell-free DNA tubes for separating different cells from the blood when used in combination with Histopaque-1077 for a double gradient?
1 answer-
The PAX gene Blood DNA tubes were not tested for compatibility with any of the Histopaque products. As a general rule, fresh blood provides the best results with the Histopaque products, and it is generally not recommended to use blood stored for more than 24 hours.
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Are there any comparable products for Pancoll at densities 1.119 g/ml and 1.077 g/ml, to be used in cell isolation for gene expression studies or diagnostics.
1 answer-
As Pancoll is polysucrose-based, Histopaque and Ficoll Paque have essentially the same formulations. For Histopaque and Ficoll Paque, products are available with densities of 1.073, 1.077, 1.083, 1.084, and 1.119 g/ml. Any of the Histopaque and Ficoll Paque products with densities of 1.077 g/ml and 1.119 g/ml should be suitable crossmatches for Pancoll products with densities of 1.077 g/ml and 1.119 g/ml.
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Is there a product similar to Mono-Poly resolving media?
1 answer-
The Mono-Poly resolving media has only a slight difference in formulation compared to Histopaque 11191, primarily in its density which can vary from 1.112 to 1.116 g/ml. In contrast, the specification for Histopaque 11191 is 1.119 g/ml +/- 0.001 g/ml.
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Is it possible to use Histopaque 1119 as a double gradient media with another brand of density gradient media, such as Ficoll paque plus, or does it only work with Histopaque 1077?
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Histopaque and Ficoll Paque are chemically identical solutions. For all practical purposes, both have polysucrose 400.
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Hi. I am trying to adapt an older protocol that calls for a certain percentage of Ficoll. What percentage does this product contain?
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This product is a mixture of Ficoll (polysucrose 400) and sodium diatrizoate at 60 grams and 167 grams per liter, respectively. The solution may not be a suitable substitute for protocols requiring Ficoll, only. Please see product F5415 to review a 20% Ficoll solution in water. This item may a better option:
https://www.sigmaaldrich.com/product/sial/f5415Other pure Ficoll products are available. See these potential product numbers below:
F4375
F2637
F8016
F9378
F8636
F1418
GE17-0300-50
GE17-0300-10
GE17-0300-05Helpful?
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Can the procedure for using Product 11191, Histopaque®-1119, be used successfully with species such as rats and mice?
1 answer-
The Histopaque 1119-1 procedure was developed for use with human blood. Due to the lower number of circulating neutrophils in rat or mouse peripheral blood, the technique tends not to work as well with rats or mice.
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Can the procedure for Product 11191, Histopaque®-1119, be performed using the various Accuspin products?
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No. Accuspin products are intended for use with just a single density gradient - such as Histopaque® 1077.
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The Product 11191, Histopaque®-1119, froze in transit. Is the product still suitable for use?
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Mix the bottles upon receipt several times to ensure the product is uniform. The products are shipped ambient and gradients in situ can form if the solution freezes during transport. As long as the bottle has not broken and no precipitate is noted, the product is suitable for use after slowly thawing and mixing.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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