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37294

Dihydrofolate Reductase from bovine liver

~8 U/mL, ammonium sulfate suspension, off-white

Synonym(s):

Tetrahydrofolate Dehydrogenase

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About This Item

CAS Number:
9002-03-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.5.1.3 (BRENDA, IUBMB)
MDL number:
MFCD00130955
Specific activity:
≥6 U/mg protein
Biological source:
bovine liver
Concentration:
~8 U/mL
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biological source

bovine liver

form

ammonium sulfate suspension

specific activity

≥6 U/mg protein

concentration

~8 U/mL

color

off-white

density

1.2 g/mL at 20 °C

storage temp.

2-8°C

Application

Dihydrofolate reductase (DHFR) is involved in the synthesis of purines, thymidine and glycine in folate metabolism. DHFR, from bovine liver, is used for methotrexate analysis . DHFR is useful to study various cancers such as sarcomas, leukemias and head and neck cancers .

Biochem/physiol Actions

Dihydrofolate reductase (DHFR) reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as an electron donor. The binding of antitumor agents, such as methotrexate, to DHFR prevents the formation of reduced folates, which are essential for DNA synthesis .

Physical form

Suspension in 3.6 M ammonium sulfate solution, pH 7.0

Other Notes

In folate metabolism for the synthesis of purines, thymidine and glycine
1 U corresponds to the amount of enzyme which converts 1 μmol 7,8-dihydrofolate and NADPH to 5,6,7,8-tetrahydrofolate and NADP per minute at pH 6.5 and 25 °C
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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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