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40839

Anti-Rabbit-IgG - Atto 647N antibody produced in goat

1 mg/mL IgG

Synonym(s):

Atto 647N-Anti-Rabbit-IgG antibody produced in goat

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1 mL
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RM 1,749.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD06798562
Conjugate:
Atto 647N conjugate
Clone:
polyclonal
Application:
IF
Citations:
66

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conjugate

Atto 647N conjugate

Quality Segment

100

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL IgG

technique(s)

immunofluorescence: suitable (5μg/ml)

fluorescence

λex 647 nm; λem 665 nm in PBS

suitability

in accordance for fluorescence

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.

Immunogen

rabbit IgG

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.Atto 647N goat anti-rabbit IgG was used as the secondary antibody for immunofluorescene at a concentration of 5μg/ml on cells fixed in 2% formaldehyde.

Physical form

Atto 647 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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77671A381243328
Sigma-Aldrich

Sigma-Aldrich

40839

Anti-Rabbit-IgG - Atto 647N antibody produced in goat

Sigma-Aldrich

Sigma-Aldrich

77671

Anti-Rabbit IgG - Atto 594 antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Rabbit-IgG - Atto 550 antibody produced in goat

Sigma-Aldrich

43328

Anti-Rabbit-IgG - Atto 550 antibody produced in goat

description

1 mg/mL IgG

description

-

description

affinity isolated antibody, buffered aqueous glycerol solution

description

-

technique(s)

immunofluorescence: suitable (5μg/ml)

technique(s)

-

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

immunofluorescence: suitable

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

conjugate

Atto 647N conjugate

conjugate

Atto 594 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

Atto 550 conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

form

liquid

form

liquid

form

buffered aqueous glycerol solution

form

liquid

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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