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Anti-Rabbit-IgG - Atto 550 antibody produced in goat

Name: Anti-Rabbit-IgG - Atto 550 antibody produced in goat
Brand: SIGMA
Price: 793 CAD

Synonym(s):

Atto 550-Anti-Rabbit-IgG antibody produced in goat

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Size/SKU	Availability	Price
1 mL	Please contact Customer Service for Availability	$793.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD06798560

Conjugate:

Atto 550 conjugate

Clone:

polyclonal

Application:

Citations:

$793.00

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Properties

conjugate

Atto 550 conjugate

Quality Segment

100

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL protein

technique(s)

immunofluorescence: suitable

fluorescence

λ_ex 550 nm; λ_em 576 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.

Immunogen

rabbit IgG

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 550-goat anti-rabbit-IgG has been used for immunofluorescence applications.

Physical form

Atto 550 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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1 of 1

This Item	77671	A0545	40839
Sigma-Aldrich 43328 Anti-Rabbit-IgG - Atto 550 antibody produced in goat	Sigma-Aldrich 77671 Anti-Rabbit IgG - Atto 594 antibody produced in goat	Sigma-Aldrich A0545 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	Sigma-Aldrich 40839 Anti-Rabbit-IgG - Atto 647N antibody produced in goat
technique(s) immunofluorescence: suitable	technique(s) -	technique(s) direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)	technique(s) immunofluorescence: suitable (5μg/ml)
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rabbit
conjugate Atto 550 conjugate	conjugate Atto 594 conjugate	conjugate peroxidase conjugate	conjugate Atto 647N conjugate
shipped in wet ice	shipped in -	shipped in dry ice	shipped in wet ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
form liquid	form liquid	form -	form liquid

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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