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Anti-Rabbit-IgG - Atto 550 antibody produced in goat

Name: Anti-Rabbit-IgG - Atto 550 antibody produced in goat
Brand: SIGMA

Synonym(s):

Atto 550-Anti-Rabbit-IgG antibody produced in goat

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD06798560

Conjugate:

Atto 550 conjugate

Clone:

polyclonal

Application:

Citations:

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Properties

conjugate

Atto 550 conjugate

Quality Level

100

antibody product type

secondary antibodies

clone

polyclonal

form

liquid

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL protein

technique(s)

immunofluorescence: suitable

fluorescence

λ_ex 550 nm; λ_em 576 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat anti-rabbit IgGs are known to associate with rabbit IgGs.

Immunogen

rabbit IgG

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 550-goat anti-rabbit-IgG has been used for immunofluorescence applications.

Physical form

Atto 550 goat anti-rabbit IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A3812	A0545	77671
Sigma-Aldrich 43328 Anti-Rabbit-IgG - Atto 550 antibody produced in goat	Sigma-Aldrich A3812 Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A0545 Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat	Sigma-Aldrich 77671 Anti-Rabbit IgG - Atto 594 antibody produced in goat
technique(s) immunofluorescence: suitable	technique(s) direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)	technique(s) -
conjugate Atto 550 conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate	conjugate Atto 594 conjugate
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rabbit
form liquid	form buffered aqueous glycerol solution	form -	form liquid
shipped in wet ice	shipped in wet ice	shipped in dry ice	shipped in -
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Global Trade Item Number

SKU	GTIN
43328-1ML-F	04061838061935

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