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HEPES

Name: HEPES
Brand: SIGMA
Price: 77.2 USD
Availability: InStock

≥99.5% (titration), powder, for molecular biology, BioUltra

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Size/SKU	Availability	Price
10 g	Estimated to ship onAugust 17, 2026fromMILWAUKEE	$77.20
50 g	Estimated to ship onAugust 17, 2026fromMILWAUKEE	$304.00
250 g	Estimated to ship onAugust 17, 2026fromMILWAUKEE	$1,030.00

About This Item

Empirical Formula (Hill Notation):

C₈H₁₈N₂O₄S

CAS Number:

7365-45-9

Molecular Weight:

238.30

NACRES:

NA.25

PubChem Substance ID:

57651473

UNSPSC Code:

12161700

EC Number:

230-907-9

MDL number:

MFCD00006158

Beilstein/REAXYS Number:

883043

eCl@ss:

32129211

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Properties

Product Name

HEPES, BioUltra, Molecular Biology, ≥99.5% (T)

grade

Molecular Biology

Quality Segment

200

product line

BioUltra

assay

≥99.5% (T)

form

powder

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

ign. residue

≤0.1% (as SO₄)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H₂O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H₂O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤50 mg/kg, sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H₂O

UV absorption

λ: 260 nm A_max: 0.045, λ: 280 nm A_max: 0.035

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:

DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
Recapture solution to re-capture RNA exosomes from the glycerol gradient
ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Easily compare specifications for HEPES products with the HEPES specification table.

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This Item	H6147	H7523	H4034
Sigma-Aldrich 54457 HEPES	Sigma-Aldrich H6147 HEPES	Sigma-Aldrich H7523 HEPES	Sigma-Aldrich H4034 HEPES
description BioUltra, Molecular Biology, ≥99.5% (T)	description BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)	description BioXtra, pH 5.0-6.5 (1 M in H₂O), ≥99.5% (titration)	description BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
assay ≥99.5% (T)	assay ≥99.5% (titration)	assay ≥99.5% (titration)	assay ≥99.5% (titration)
grade Molecular Biology	grade -	grade -	grade BioPerformance Certified
product line BioUltra	product line BioXtra	product line BioXtra	product line -
Quality Level 200	Quality Level 400	Quality Level 400	Quality Level 400
form powder	form powder	form powder	form crystalline powder
solubility H₂O: 1 M at 20 °C, clear, colorless	solubility water: 500 mg/mL, clear, colorless	solubility H₂O: 1 M, clear, colorless	solubility -

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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c