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Phosphate Buffered Saline

Name: Phosphate Buffered Saline
Brand: SIGMA

pH 7.4, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Phosphate buffered saline, PBS

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00131855

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Properties

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

Description

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate-buffered saline has been used:

as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
has also been used to wash cells during immunofluorescence staining
as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)

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This Item	P4474	P2272	P4244
SAFC 806552 Phosphate Buffered Saline	Sigma-Aldrich P4474 Phosphate buffered saline	Sigma-Aldrich P2272 Phosphate buffered saline	Sigma-Aldrich P4244 Phosphate buffered saline
description pH 7.4, liquid, sterile-filtered, suitable for cell culture	description pH 7.4, liquid, sterile-filtered, suitable for cell culture	description pH 7.2, liquid, sterile-filtered, suitable for cell culture	description Autoclaved, pH 7.2 (25 °C)
form liquid	form liquid	form liquid	form liquid
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) -
pH 7.4	pH 7.4	pH 7.2	pH 7.2 (25 °C)
impurities Endotoxin, tested	impurities Endotoxin, tested	impurities Endotoxin, tested	impurities -
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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