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806552

Phosphate Buffered Saline

pH 7.4, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Phosphate buffered saline, PBS

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1 L
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$76.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00131855

$76.00

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sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate-buffered saline has been used:
  • as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
  • has also been used to wash cells during immunofluorescence staining[1]
  • as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)

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This Item
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Phosphate Buffered Saline pH 7.4, liquid, sterile-filtered, suitable for cell culture

SAFC

806552

Phosphate Buffered Saline

Phosphate Buffered Saline pH 7.2, liquid, sterile-filtered, suitable for cell culture

SAFC

806544

Phosphate Buffered Saline

Phosphate Buffered Saline 10× PBS for Western blots and IP

Sigma-Aldrich

P7059

Phosphate Buffered Saline

Phosphate buffered saline pH 7.2 (25 °C)

Sigma-Aldrich

P5119

Phosphate buffered saline

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

-

impurities

Endotoxin, tested

impurities

Endotoxin, tested

impurities

-

impurities

-

pH

7.4

pH

7.2

pH

7.2-7.6(1x solution)

pH

7.2 (25 °C)

sterility

sterile-filtered

sterility

sterile-filtered

sterility

0.2 μm filtered

sterility

autoclaved

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL9234
RNBL6557
RNBL5991
RNBL4440
RNBL3279

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Protocols

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

View All Protocols

Articles

Cellular Fluorescence Imaging with Millicell® Cell Culture Inserts

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

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Guide technique : test de prolifération et de viabilité cellulaires au WST-1

Protocole du test au WST-1 pour la mesure de la viabilité, de la prolifération, de l'activation et de la toxicité cellulaires. Instructions de préparation du réactif WST-1 et exemples d'applications. Foire aux questions et guide de résolution des problèmes liés au test au WST-1.

Shangang Zhao et al.
Molecular metabolism, 37, 100995-100995 (2020-04-15)
Hyperleptinemia per se is sufficient to promote leptin resistance in the obese state. Leptin sensitivity can be restored by reducing circulating leptin levels within a physiologically healthy range and is a viable antiobesity and antidiabetic strategy. However, a previous study
Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
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Molecular Neurobiology (2016)
Pinkal D Patel et al.
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Chronic elevation of intraocular pressure (IOP) is a major risk factor associated with primary open angle glaucoma (POAG), a common form of progressive optic neuropathy that can lead to debilitating loss of vision. Recent studies have identified the role of
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Global Trade Item Number

SKUGTIN
806552-500ML04061837505911
806552-1L04061832983288

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