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MilliporeSigma

92544

Abberior® FLIP 565, maleimide

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352111
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Quality Segment

form

solid

concentration

≥50.0% (degree of coupling)

solubility

DMF: 0.25 mg/mL, clear

fluorescence

λex 565 nm; λem 580 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum (off-state) λmax:314 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 47,000 M-1cm-1 (MeOH)
Fluorescence Maximum, λfl:580 nm (PBS, pH 7.4)
Photoactication Wavelength: 310-380 (one-photon activation)
650-800 (two-photon activation)
Fluorescence Quantum Yield, η: 0.38 (PBS, pH 7.4)

Application

Abberior® FLIP 565 conjugated with secondary antibody has been used for STORM (stochastic optical reconstruction microscopy) imaging of COS-7 and S180 cells.
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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