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C0150

Cathepsin B from human placenta

lyophilized powder, ≥5 units/mg protein

Synonym(s):

Cathepsin B1

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About This Item

CAS Number:
9047-22-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.22.1 (BRENDA, IUBMB)
MDL number:
MFCD00130748
Specific activity:
≥5 units/mg protein

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form

lyophilized powder

specific activity

≥5 units/mg protein

mol wt

24.5 kDa

composition

Protein, ~50% Lowry

solubility

H2O: soluble 1.0 mg/mL, clear

UniProt accession no.

P07858

storage temp.

−20°C

Quality Level

200

Gene Information

human ... CTSB(1508)

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This Item
C8571SRP0289219362
Cathepsin B from human placenta lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

C0150

Cathepsin B from human placenta

Cathepsin B from human liver buffered aqueous solution, ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)

Sigma-Aldrich

C8571

Cathepsin B from human liver

Cathepsin B Active human recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Sigma-Aldrich

SRP0289

Cathepsin B Active human

Cathepsin B, Human Liver Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

Sigma-Aldrich

219362

Cathepsin B, Human Liver

specific activity

≥5 units/mg protein

specific activity

≥1,500 units/mg protein (E1%/280)

specific activity

≥2228 pmol/min-μg

specific activity

≥10 units/mg protein

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSB(1508)

Gene Information

human ... CTSB(1508)

form

lyophilized powder

form

buffered aqueous solution

form

aqueous solution

form

liquid

solubility

H2O: soluble 1.0 mg/mL, clear

solubility

-

solubility

water: soluble

solubility

-

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

UniProt accession no.

P07858

UniProt accession no.

P07858

UniProt accession no.

-

UniProt accession no.

-

General description

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1.[1] Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.[2]

Application

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG).[3] It has also been used in determining the effect of cysteine protease inhibitors.[2]
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease [4]. Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Biochem/physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments [4]. Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer [5].

Physical form

Lyophilized powder containing phosphate buffer salts

Preparation Note

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Other Notes

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
014K37832
104K3781
116H38382
116H38381
081H38161

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Scavenger Receptor A Mediates the Clearance and Immunological Screening of MDA-Modified Antigen by M2-Type Macrophages
Warnecke A, et al.
Neuromolecular Medicine, 19(4), 463-479 (2017)
Sarah Mueller-Steiner et al.
Neuron, 51(6), 703-714 (2006-09-20)
Alzheimer's disease (AD) may result from the accumulation of amyloid-beta (Abeta) peptides in the brain. The cysteine protease cathepsin B (CatB) is associated with amyloid plaques in AD brains and has been suspected to increase Abeta production. Here, we demonstrate
Mixed gastric carcinomas show similar chromosomal aberrations in both their diffuse and glandular components.
Carvalho B, et al.
Analytical Cellular Pathology, 28(5-6), 283-294 (2006)
Cathepsins B, H, L and cysteine protease inhibitors in malignant prostate cell lines, primary cultured prostatic cells and prostatic tissue
Friedrich B, et al.
European Journal of Cancer, 35(1), 138-144 (1999)
Viktorija Herceg et al.
Molecules (Basel, Switzerland), 25(18) (2020-09-24)
Cyclopeptidic chemotherapeutic prodrugs (cPCPs) are macromolecular protease-sensitive doxorubicin (DOX) prodrugs synthesized from a cyclodecapeptidic scaffold, termed Regioselectively Addressable Functionalized Template (RAFT). In order to increase the chemotherapeutic potential of DOX and limit its toxicity, we used a Cathepsin B (Cat
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Articles

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Related Content

Product Information Sheet - C0150

Product Information Sheet

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