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Anti-Calreticulin antibody produced in rabbit

Name: Anti-Calreticulin antibody produced in rabbit
Brand: SIGMA
Price: 495.55 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$583.00 $495.55

About This Item

UNSPSC Code:

12352203

NACRES:

NA.43

MDL number:

MFCD01092536

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, canine

technique(s)

immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, microarray: suitable, western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line

UniProt accession no.

P27797

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALR(811)

Description

General description

The gene for calreticulin (CALR) is located on the human chromosome 19p13.13. The encoded protein is a predominantly conserved calcium-binding chaperone, which is localized primarily to the lumen of the endoplasmic reticulum (ER). Calreticulin comprises three domains, a globular N-domain, a proline-rich P-domain, and a highly acidic C-domain.

Immunogen

synthetic peptide corresponding to the C-terminus of human calreticulin (amino acids 401-417).

Application

Anti-Calreticulin antibody produced in rabbit has been used in:

immunoblotting[1]
immunofluorescence[2]
immunocytochemistry{91
immunoprecipitation

Biochem/physiol Actions

Anti-Calreticulin recognizes human and dog calreticulin (55-60 kDa).

Calreticulin facilitates transient interaction with newly synthesized cellular and extracellular proteins for folding and assembling in the endoplasmic reticulum (ER) before its localization to the cytosol or cell surface. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins and misfolded proteins. It is believed to play a critical role in quality control processes during protein synthesis and folding and calcium (Ca²⁺) homeostasis. Increased expression of calreticulin increases the Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺-influx and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺-adenosine triphosphatase (ATPase) (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. However, increased resistance to apoptosis has been observed in calreticulin-deficient cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Store at -20 °C. For continuous use, the product maybe stored at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at-20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded ifnot used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C3237	C7488	E3406
$Anti-Calreticulin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C4606 Anti-Calreticulin antibody produced in rabbit	$Anti-Caveolin-1 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C3237 Anti-Caveolin-1 antibody produced in rabbit	$Anti-CENP-E antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C7488 Anti-CENP-E antibody produced in rabbit	Sigma-Aldrich E3406 Anti-EB1 antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) immunoprecipitation (IP): suitable using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line, microarray: suitable, indirect immunofluorescence: 1:100 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated, Madin Darby canine kidney MDCK cell line, western blot: 1:2,000 using whole cell RIPA lysate of the human epitheloid carcinoma HeLa cell line	technique(s) indirect immunofluorescence: 1:500 using mouse fibroblast NIH3T3 cell line, microarray: suitable, western blot: 1:2,000 using whole cell extract of the human umbilical vein endothelial HUVEC cell line and the rat fibroblast Rat-1 cell line	technique(s) immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line., microarray: suitable, western blot: 1:2,000 using whole extract of HeLa nuclear cell line	technique(s) immunoprecipitation (IP): 10-20 μg using cell lysate of the human epitheloid carcinoma HeLa cell line, indirect immunofluorescence: 1-2 μg/mL using rat fibroblast Rat1 cells, indirect immunofluorescence: suitable, western blot: 0.5-1 μg/mL using cell lysate of the rat fibroblast cell line Rat1 or Rat 2
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Clinical and hematological relevance of JAK2 V617F and CALR mutations in BCR-ABL-negative ET patients.

N Limsuwanachot et al.

Hematology (Amsterdam, Netherlands), 22(10), 599-606 (2017-04-14)

Classical BCR-ABL1-negative myeloproliferative neoplasms (MPNs) including polycythemia vera, essential thrombocythemia (ET), and primary myelofibrosis frequently harbor JAK2, MPL, and CALR somatic mutations. AS-PCR for JAK2 V617F, pyrosequencing for MPL W515L/K, and PCR-fragment analysis for CALR exon 9 mutations were established

CBFB-SMMHC is correlated with increased calreticulin expression and suppresses the granulocytic differentiation factor CEBPA in AML with inv(16).

Daniel Helbling et al.

Blood, 106(4), 1369-1375 (2005-04-28)

The pericentric inversion of chromosome 16, inv(16)(p13q22), is associated with acute myeloid leukemia (AML) subtype M4Eo that is characterized by the presence of myelomonocytic blasts and atypical eosinophils. This rearrangement fuses the CBFB and MYH11 genes, with the latter encoding

Upon Infection the Cellular WD Repeat-containing Protein 5 (WDR5) Localizes to Cytoplasmic Inclusion Bodies and Enhances Measles Virus Replication.

Dzwokai Ma et al.

Journal of virology (2017-12-15)

Replication of negative-strand RNA viruses occurs in association with discrete cytoplasmic foci called inclusion bodies. Whereas inclusion bodies represent a prominent subcellular structure induced by viral infection, our knowledge of the cellular protein components involved in inclusion body formation and

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Global Trade Item Number

SKU	GTIN
C4606-.2ML	04061838104335

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