D3818
Dextran Ladder
Glycan standard for HPLC
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About This Item
UNSPSC Code:
12352201
biological source
bacterial (Leuconostoc mesenteroides)
form
solid
shipped in
wet ice
storage temp.
−20°C
Application
The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. This standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide, dependent upon the chromatographic conditions employed. The elution position of each peak in this ladder is expressed as a glucose unit (gu) and is used to assign gu values to peaks in the released glycan pool.Dextran ladder upon derivitization can be used as a new tool to assess cell layer tightness.
Preparation Note
The dextran ladder is prepared by partial acid hydrolysis of dextran from Leuconostoc mesenteroides with an average molecular weight of 100-200 kDa. The purity and structural integrity of the ladder is assessed by fluorescently labeling an aliquot and subsequent analysis by normal phase HPLC.
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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