Name: Enhanced Avian HS RT-PCR Kit
Brand: SIGMA
Price: 858.5 USD

Quality Level

200

usage

sufficient for 100 reactions

feature

dNTPs included, hotstart

technique(s)

RT-PCR: suitable, cDNA synthesis: suitable

color

colorless

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

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PCR & Amplification

PCR Reagents & Kits

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This Item	STR1	QR0100	TOSRTRO
Sigma-Aldrich HSRT100 Enhanced Avian HS RT-PCR Kit	Sigma-Aldrich STR1 Enhanced Avian First Strand Synthesis Kit	Sigma-Aldrich QR0100 SYBR^® Green Quantitative RT-qPCR Kit	Roche TOSRTRO Transcriptor One-Step RT-PCR Kit
technique(s) RT-PCR: suitable, cDNA synthesis: suitable	technique(s) RT-PCR: suitable, cDNA synthesis: suitable	technique(s) RT-qPCR: suitable	technique(s) RT-PCR: suitable, RT-qPCR: suitable
color colorless	color colorless	color colorless	color -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
input purified RNA	input purified RNA	input purified RNA	input purified RNA
usage sufficient for 100 reactions	usage sufficient for 50 reactions, kit sufficient for 50 reactions	usage sufficient for 250 reactions	usage sufficient for 150 reactions, sufficient for 50 reactions
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in -

General description

Procedures are provided for one-step and two-step RT-PCR reactions.

One-step: In a single tube, eAMV^™ RT and AccuTaq LA act sequentially to first produce cDNA and then immediately amplify by PCR. This provides quick, sensitive analysis of RNA.

Two-step: Each reaction is individually optimized for greater yields with high fidelity, when protocol requires multiple amplifications, or if maximum yield is more important than maximum convenience.

Application

Enhanced Avian HS RT-PCR Kit has been used to synthesize the cDNA first-strand during reverse transcriptase PCR (RT-PCR) analysis.[1][2]

Features and Benefits

Greater transcription lengths than other reverse transcriptases, generating cDNA up to 14.1 Kb.
Higher sensitivity for detecting low abundance messages. eAMV^™ RT is able to transcribe RNA that other reverse transcriptases cannot detect.
Unsurpassed transcription through difficult secondary structure.
Increased sensitivity, specificity and yield from JumpStart AccuTaq LA DNA Polymerase.

Other Notes

Reverse Transcriptase PCR (RT-PCR) is a powerful tool used to study gene expression. The Enhanced Avian HS RT-PCR kit utilizes an enhanced avian myeloblastosis virus reverse transcriptase (eAMV-RT) enzyme that offers superior performance in comparison to standard AMV-RT and Moloney murine leukemia virus reverse transcriptase (MMLV-RT). eAMV RT is an exceptionally robust enzyme with an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA (up to 14.1 kb) from total RNA or poly(A)⁺ RNA. JumpStart^™ AccuTaq^™ LA DNA polymerase mix is also provided to eliminate non-specific amplification and increase specificity and sensitivity. The combination of these two enzymes provides a quality system that offers the versatility of a one-step or two-step RT-PCR protocol.

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

JumpStart is a trademark of Sigma-Aldrich Co. LLC

eAMV is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.

Description

SDS

O4387Random nonamers 100 μLSDS

O4387Anchored oligo(dT)₂₃ primers 100 μLSDS

B017410x reaction buffers 10x AccuTaq bufferSDS

P219210 mM dNTP mix 10 x PCR bufferSDS

W1754Nuclease-free water 4 x 1.5SDS

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

signalword

Danger

wgk

WGK 3

hcodes

H412,EUH430

pcodes

P201 - P202 - P273 - P391 - P405 - P501

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1

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West Nile virus infection alters midgut gene expression in Culex pipiens quinquefasciatus Say (Diptera: Culicidae).

Chelsea T Smartt et al.

The American journal of tropical medicine and hygiene, 81(2), 258-263 (2009-07-29)

Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding

Transgenic chickpea expressing a recombinant human $\alpha$ 1-proteinase inhibitor ($\alpha$ 1-PI) driven by a seed-specific promoters from the common bean Phaseolus vulgaris (L.)

Mishra S, et al.

Plant Cell, Tissue and Organ Culture, 115(1), 23-33 (2013)

Convergent sets of data from in vivo and in vitro methods point to an active role of Hsp60 in chronic obstructive pulmonary disease pathogenesis.

Cappello F, et al.

PLoS ONE, 6, e28200-e28200 (2011)

Micafungin-Induced Cell Wall Damage Stimulates Morphological Changes Consistent with Microcycle Conidiation in Aspergillus nidulans.

Samantha Reese et al.

Journal of fungi (Basel, Switzerland), 7(7) (2021-07-03)

Fungal cell wall receptors relay messages about the state of the cell wall to the nucleus through the Cell Wall Integrity Signaling (CWIS) pathway. The ultimate role of the CWIS pathway is to coordinate repair of cell wall damage and

Micafungin-Induced Cell Wall Damage Stimulates Morphological Changes Consistent with Microcycle Conidiation in Aspergillus nidulans.

Samantha Reese et al.

Journal of fungi (Basel, Switzerland), 7(7) (2021-07-03)

Fungal cell wall receptors relay messages about the state of the cell wall to the nucleus through the Cell Wall Integrity Signaling (CWIS) pathway. The ultimate role of the CWIS pathway is to coordinate repair of cell wall damage and

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The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Global Trade Item Number

SKU	GTIN
HSRT100-1KT	04061833826546

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Enhanced Avian HS RT-PCR Kit

Flexible kit for one-step or two-step RT-PCR

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About This Item

HSRT100-1KT

$858.50

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