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P0101

Anti-Profilin 2 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PFN2

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Citations:
6
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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain

UniProt accession no.

P35080

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PFN2(5217)
mouse ... Pfn2(18645)
rat ... Pfn2(81531)

General description

Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein.

Immunogen

synthetic peptide corresponding to amion acid residues 128-140 of human profilin 2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting.

Biochem/physiol Actions

Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

species reactivity

human, rat

species reactivity

rat, human, mouse, chicken

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain

technique(s)

immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate, indirect immunofluorescence: 20-30 μg/mL using differentiated mouse C2 cells, western blot (chemiluminescent): 0.5-1 μg/mL using extracts of rat or mouse brain mitochondria

technique(s)

western blot: 1:1,000 using rat brain cytosol extract, western blot: 1:1,000 using whole extract of the human epitheloid carcinoma HeLa cell line

technique(s)

indirect immunofluorescence: 1:500 using mouse fibroblasts NIH3T3 cell line, western blot: 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of the human epidermoid carcinoma A431 cell line

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
078M4780V
078M4780V
117K4827

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Related Content

Data Sheet - P0101

Instructions

Mouse profilin 2 regulates endocytosis and competes with SH3 ligand binding to dynamin 1
Gareus R, et al.
The Journal of biological chemistry, 281(5), 2803-2811 (2006)
Profilin2 contributes to synaptic vesicle exocytosis, neuronal excitability, and novelty-seeking behavior
Boyl PP, et al.
The Embo Journal, 26(12), 2991-2991 (2007)
Lisa Marie Walter et al.
Scientific reports, 11(1), 10287-10287 (2021-05-15)
Nuclear and cytoplasmic actin-cofilin rods are formed transiently under stress conditions to reduce actin filament turnover and ATP hydrolysis. The persistence of these structures has been implicated in disease pathology of several neurological disorders. Recently, the presence of actin rods
View All Related Papers

Global Trade Item Number

SKUGTIN
P0101-25UL04061837721274
P0101-200UL04061837721267

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