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Anti-Profilin 2 (C-terminal) antibody produced in rabbit

Name: Anti-Profilin 2 (C-terminal) antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PFN2

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

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Properties

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain

UniProt accession no.

P35080

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PFN2(5217)
mouse ... Pfn2(18645)
rat ... Pfn2(81531)

Description

General description

Profilins are actin-monomer-binding proteins, abundantly expressed in all eukaryotic cells from yeast to mammals. Profilin 2 (PFN2) is the neuronal specific isoform. It is an actin-binding protein and is a member of the small G-actin-binding class of protein.

Immunogen

synthetic peptide corresponding to amion acid residues 128-140 of human profilin 2 conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-Profilin 2 (C-terminal) antibody produced in rabbit has been used in immunostaining and immunoblotting.

Biochem/physiol Actions

Profilins are key regulators of actin dynamics. Profilin 2 (PFN2) associates with a number of proteins involved in membrane trafficking and endocytosis, such as synapsin and dynamin 1. It associates with dynamin 1 through the C-terminal proline-rich domain of dynamin 1 and thereby competes with the binding of SH3 ligands, such as endophilin and amphiphysin, thus interfering with the assembly of the endocytic machinery. Overexpression of profilin 2 inhibits endocytosis. Absence of profilin 2 in neurons increases endocytosis and membrane recycling. Profilin 2 is required for stimulated actin polymerization in the synapse and is involved in the regulation of synaptic vesicle exocytosis, neuronal excitability and complex behavior.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200357	C8992	WH0005217M4
$Anti-Profilin 2 (C-terminal) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich P0101 Anti-Profilin 2 (C-terminal) antibody produced in rabbit	Sigma-Aldrich SAB4200357 Anti-Profilin 1 antibody, Mouse monoclonal	$Anti-phospho-Cofilin (pSer3) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich C8992 Anti-phospho-Cofilin (pSer³) antibody produced in rabbit	Sigma-Aldrich WH0005217M4 Monoclonal Anti-PFN2 antibody produced in mouse
biological source rabbit	biological source mouse	biological source rabbit	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form purified from hybridoma cell culture	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin
clone polyclonal	clone Profilin 1-3, monoclonal	clone polyclonal	clone 5F11, monoclonal
technique(s) western blot: 1:500-1:1,000 using whole extracts of human SH-SY5Y cells, mouse brain, and rat brain	technique(s) immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using whole extracts of HeLa or NRK cells	technique(s) western blot: 1:2,000-1:4,000 using whole extracts of HeLa human epithelioid carcinoma and mouse NIH3T3 cells	technique(s) indirect ELISA: suitable, western blot: 1-5 μg/mL
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

078M4780V

117K4827

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Global Trade Item Number

SKU	GTIN
P0101-25UL	04061837721274
P0101-200UL	04061837721267

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