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PCR9604

GenElute 96 Well PCR Clean-Up Kit

4 x 96 well plate, sufficient for 4 96-well plate purifications

Synonym(e):

High Throughput Purification, PCR Purification, PCR Purification, PCR Purification Kit

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Ihnen/SKUVerfügbarkeitPreis
1 kit
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€ 963,00
€ 818,55

Über diesen Artikel

NACRES:
NA.52
UNSPSC Code:
41106300

€ 818,55

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usage

sufficient for 4 96-well plate purifications

quality

4 x 96 well plate

technique(s)

DNA purification: suitable

input

sample(s) (PCR)

test parameters

: <45 min kit run time, sample volume: 100 μL

storage temp.

15-25°C

General description

GenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 bp to 10 kb and removes primers, primer-dimers, nucleotides, salts and polymerase.

Once the PCR reaction is complete, the reaction volume is adjusted and Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides, and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation, and microarray analysis.

Application

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation and microarray analysis.

Biochem/physiol Actions

Once the PCR reaction is complete, the reaction volume is adjusted and the Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.

Features and Benefits

  • Complete removal of primers and primer-dimers
  • Innovative wash plate minimizes risk of cross-contamination
  • Suitable for processing under vacuum and centrifugation
  • Time-saving parallel clean-up of PCR products

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Dieser Artikel
NA1020NA9604NA1111
technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

usage

sufficient for 4 96-well plate purifications

usage

sufficient for 70 purifications

usage

sufficient for 4 96-well plate purifications

usage

sufficient for 70 purifications

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

test parameters

: <45 min kit run time, sample volume: 100 μL

test parameters

sample volume: 10 μg, sample volume: 100 μL

test parameters

: <50 min kit run time, sample volume: 1.3 mL bacterial culture

test parameters

sample volume: 3.5 g agarose

input

sample(s) (PCR)

input

DNA (single or double stranded PCR ampliflied)

input

-

input

sample(s) (gel slices)

quality

4 x 96 well plate

quality

-

quality

-

quality

-


Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • Binding Solution 2

  • Wash Solution, Concentrate

  • Elution Solution

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

Lagerklasse

8A - Combustible corrosive hazardous materials

wgk

WGK 3



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