RNA Marker sample solutions for electrophoresis should be prepared as follows: Mix 2-4 µl of RNA Marker, add water (Rnase free) to make up to 5 µl, then add 3 µl of RNA Sample Loading Buffer, and 1 µl of 200 mM Potassium Acetate, pH 4.5. Additional information can be found in the Product Information Sheet.
R7020
Transcript RNA Markers 0.2-10 kb
for RNA electrophoresis
Synonym(s):
RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker
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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Please contact Customer Service for Availability | $269.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
41105337
Form:
liquid
Solubility:
water: soluble
Storage temp.:
−70°C
$269.00
Please contact Customer Service for Availability
grade
Molecular Biology
Quality Segment
form
liquid
usage
25 uses
solubility
water: soluble
suitability
suitable for electrophoresis (RNA)
storage temp.
−70°C
General description
Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Application
Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.
Other Notes
Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.
Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
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This Item | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| solubility water: soluble | solubility - | solubility - | solubility water: soluble |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for electrophoresis (RNA) | suitability suitable for electrophoresis (RNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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in what proportion should i mix RNA marker and loading dye buffer.
1 answer-
Helpful?
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Can I denaturate it using Glyoxal?
1 answer-
This product has been tested on 1% agarose gel with formaldehyde. It has not been specifically qualified for use with glyoxal, however there is no reason to suspect that it would not be suitable. See the reference in the link below comparing formaldehyde to glyoxal:
https://link.springer.com/protocol/10.1385/0-89603-127-6:1Helpful?
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