This product has been dried during the production process. The quality of the material following multiple drying cycles has not been investigated. To assure optimum quality, the purchase of fresh product is recommended. The current drying conditions are as follows:
Jacket temperature - 65 °C
Product optimum temperature - 33 - 38°C
Drying time under continuous mixing - 3 to 6 hours
End point loss on drying <0.2%
T1503
Trizma® base
≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate
Trizma® Base
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 g | Available to ship TODAYfromMILWAUKEE | $28.20 | |
| 100 g | Available to ship TODAYfromMILWAUKEE | $43.40 | |
| 250 g | Available to ship TODAYfromMILWAUKEE | $79.40 | |
| 500 g | Available to ship TODAYfromMILWAUKEE | $120.00 | |
| 1 kg | Available to ship TODAYfromMILWAUKEE | $197.00 | |
| 5 kg | Available to ship TODAYfromMILWAUKEE | $834.00 | |
| 10 kg | Available to ship TODAYfromMILWAUKEE | $1,380.00 | |
| 25 kg | Available to ship TODAYfromMILWAUKEE | Inquire | |
| 25 kg | Available to ship TODAYfromMILWAUKEE | Inquire | |
| 50 kg | Available to ship TODAYfromMILWAUKEE | Inquire |
About This Item
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12352104
EC Number:
201-064-4
MDL number:
Beilstein/REAXYS Number:
741883
$28.20
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Product Name
Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
Quality Level
description
aminopeptidase substrate
assay
≥99.9% (titration)
form
crystalline
storage condition
dry at room temperature
technique(s)
ELISA: suitable, protein extraction: suitable
color
white
pH
10.5-12
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
methanol: soluble 26 mg/mL at 25 °C, ethylene glycol: soluble 79.1 mg/mL at 25 °C, water: soluble (678 g/l at 20 °C)
absorption
≤0.05 at 290 nm at 40%
suitability
suitable for Western blot, suitable for electrophoresis
application(s)
advanced drug delivery
cell analysis
diagnostic assay manufacturing
life science and biopharma
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
General description
Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma® base, serves a pivotal role in diverse research applications as a biological buffer. Its optimal pKa of 8.1 makes it a preferred choice for formulating buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), ensuring the maintenance of pH within the physiological range (pH 7 - 9), applicable to a wide spectrum of living organisms. Despite its utility, researchers need to exercise caution in protein studies, as Tris has the potential to interfere with the activity of specific enzymes.
Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.
Tris base may find application as basimetric standard, independently as a buffer and as a crucial component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, TBE buffer, among others. Its attributes include purity, essential stability, and a relative non-hygroscopic nature, making it a dependable choice in laboratory settings. In these environments, Tris base is indispensable for preparing buffers compatible with biological fluids and serves as a standard pH solution. It facilitates various laboratory procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. The versatility of Tris base extends to cell biology, biochemistry, and protein research contributing significantly to studies involving cell membrane permeability and buffer preparation.
Application
Trizma® base has been used:
- as a component of H buffer (cell dissociation buffer)
- for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique[1]
- as an assay buffer for reconstitution of extracted and dried protein samples[2]
- to prepare Tris-HCl buffer that is used to stabilize proteins
- as a buffer to extract carotenoid from tubers
- as a component of sample buffer during protein extraction prior to western blotting[3]
- as a component of sample buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay
- as a buffer for polydopamine (PDA) deposition on stainless steel (SS) substrate
Features and Benefits
- Efficient buffering within the pH range of 7 - 9 with a pKa of 8.1 (25 °C)
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Can be used in Cell Biology, and Biochemical research
Packaging
The DRYPOUR® packaging system for high-quality hygroscopic salts DRYPOUR® packaging:
- The current DRYPOUR® packaging configuration is in a 50L nestable drum
- 25KG-DP are the standard prepack offering
- Smaller volumes can be put in the same drum for variable configurations
- Improves operational efficiency by reducing material preparation time and increasing operator safety
- Minimizes caking of hygroscopic salts
- Offers triple protection: against moisture inside and outside, and against contamination
- This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
- PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
- Desiccant bags are firmly integrated into the polyethylene liner
- Triple protection delivers two invaluable results:
- Dramatically reduced caking
- No contamination risk from the desiccant
Other Notes
For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
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This Item | |||
|---|---|---|---|
| assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% |
| technique(s) ELISA: suitable, protein extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 100 |
| form crystalline | form crystalline | form crystalline | form powder |
| application(s) advanced drug delivery | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| pH 10.5-12 | pH 10.5-12.0 (1 M in H2O) | pH 10.0-11.5 | pH 10.5-12, 7-9 |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Protocols
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.
To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.
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To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
Osteoprotective action of low-salt diet requires myeloid cell?derived NFAT5
Schroder A, et al.
JCI insight, 4(23) (2019)
F Foudrinier et al.
Journal of clinical microbiology, 41(4), 1681-1686 (2003-04-12)
The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adults; pregnant
Tribological performance of polydopamine + Ag nanoparticles/PTFE thin films
Choudhury D, et al.
Tribology International, 144 (2020)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T1503-1KG | 04061835266074 |
| T1503-25KG-DP | 04065267035871 |
| T1503-5KG | 04061835266111 |
| T1503-10KG | 04061835266067 |
| T1503-25KG | 04061832552279 |
| T1503-500G | 04061835266104 |
| T1503-50KG | 04061832552309 |
| T1503-250G | 04061835266081 |
| T1503-100G | 04061835266050 |
| T1503-25G | 04061835266098 |
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Can you dry this material? (sort of like KHP). Or is it better just to buy another bottle.
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this product explosive in nature?
1 answer-
As per the SDS of T1503: https://www.sigmaaldrich.com/sds/sigma/t1503?userType=anonymous, no GHS pictogram for explosive materials has been listed in Section 2, indicating it is not explosive in nature.
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How can I use T1503 to make a Trizma® buffered solution?
1 answer-
Trizma base may be used directly in preparing a buffer by adjusting the pH with a 0.1 to 1.0 M HCl or blended with Trizma hydrochloride.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What type of electrode should I use to measure the pH of Trizma® base T1503?
1 answer-
We recommend the use of glass-calomel electrodes for use with Trizma base solutions through a pH range of 0-12 and a temperature range of -5 °C to 80 °C. Silver/silver chloride reference electrodes are not recommended for use with Trizma solutions that contain proteins.
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Can Product T1503, Trizma® base be used as a standard?
1 answer-
Trizma base meets the requirements for a primary basimetric standard.
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What is the pH of Trizma® base, T1503?
1 answer-
The pH of a 0.1 M aqueous solution of Trizma base will have a pH of approximately 10.4.
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Can I autoclave solutions that contain Product T1503, Trizma® base?
1 answer-
Yes, Trizma buffered solutions may be autoclaved.
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