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A1052

Anti-Gliadin (Wheat)–Peroxidase Conjugate antibody produced in rabbit

fractionated antiserum, PBS solution

Synonym(s):

Anti-Gliadin (Wheat)

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1 mL

Available to ship TODAYfromMILWAUKEE

$701.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162321
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d)
Citations:
4

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biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS solution

species reactivity

oats, rye, soy, barley, wheat

should not react with

rice, potato

technique(s)

direct ELISA: 1:1,000, dot blot: 1:500, immunoblotting: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

wheat ... LOC543191(543191), LOC543191(543191), Loc543191(543191)

General description

Gliadin is a class of glutamine containing, alcohol soluble proteins separable from wheat and rye glutens known as the prolamins.

Immunogen

native and heat-treated wheat gliadin

Application

Rabbit anti-gliadin (wheat)-peroxidase conjugate antibody can be used for immunoblot and ELISA(1:1000).

Biochem/physiol Actions

Gliadin is a class of glutamine containing, alcohol soluble proteins separable from wheat and rye glutens known as the prolamins. These proteins are associated with the harmful effects of celiac disease and gluten sensitive enteropathy in humans, causing characteristic changes in the small intestinal mucosa. In such cases, strict compliance to a gluten-free diet is usually recommended.
Gliadin proteins are associated with the harmful effects of celiac disease and gluten sensitive enteropathy in humans, causing characteristic changes in the small intestinal mucosa. In such cases, strict compliance to a gluten-free diet is usually recommended.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Gliadin (Wheat)–Peroxidase Conjugate antibody produced in rabbit fractionated antiserum, PBS solution

Sigma-Aldrich

A1052

Anti-Gliadin (Wheat)–Peroxidase Conjugate antibody produced in rabbit

Anti-Gliadin (Wheat) antibody produced in rabbit fractionated antiserum, buffered aqueous solution

Sigma-Aldrich

G9144

Anti-Gliadin (Wheat) antibody produced in rabbit

Anti-Gliadin antibody, Mouse monoclonal clone GLD7, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200864

Anti-Gliadin antibody, Mouse monoclonal

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A7340

Anti-Glutathione-S-Transferase (GST)–Peroxidase Conjugate antibody produced in rabbit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

antibody form

fractionated antiserum

antibody form

fractionated antiserum

antibody form

purified from hybridoma cell culture

antibody form

IgG fraction of antiserum

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

unconjugated

conjugate

peroxidase conjugate

biological source

rabbit

biological source

rabbit

biological source

-

biological source

rabbit

technique(s)

direct ELISA: 1:1,000, immunoblotting: suitable, dot blot: 1:500

technique(s)

dot blot: 1:1,500, indirect ELISA: 1:5,000

technique(s)

ELISA: 0.12-0.06 μg/mL using wheat gliadin (Sigma #G3375), immunoblotting: 1-2 μg/mL using wheat gliadin (Sigma #G3375)

technique(s)

direct ELISA: 1:10,000, western blot: 1:10,000 using lysates of Escherichia coli induced to express recombinant GST

form

PBS solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000543494
0000508801
0000543494
0000508801
0000424106

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Evaluation of the applicability of two different immunoassays for the detection of wheat gluten in baby foods.
Avila, C., & Quesada, A. R.
Biochemistry and Molecular Biology Education, 28(5), 261-264 (2000)
W Weiss et al.
Electrophoresis, 14(8), 805-816 (1993-08-01)
In order to identify and characterize the wheat grain allergens involved in bakers' asthma, proteins were sequentially extracted from whole-meal flour. The polypeptide composition of the individual solubility fractions (albumin/globulin, gliadin and glutenin) was analyzed by sodium dodecyl sulfate-gel electrophoresis
Protein characteristics that affect the quality of vital wheat gluten to be used in baking: A review
Ortolan F and Steel CJ
Comprehensive Reviews in Food Science and Food Safety, 16(3), 369-381 (2017)
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Global Trade Item Number

SKUGTIN
A1052-.5ML04061835804818
A1052-1ML04061833334720

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