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A2230

Apyrase from potatoes

Name: Apyrase from potatoes
Brand: SIGMA

High Activity, ATPase ≥600 units/mg protein, lyophilized powder

Synonyme(s) :

Adénosine 5′-diphosphatase, Adénosine 5′-triphosphatase

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A propos de cet article

Numéro CAS:

9000-95-7

UNSPSC Code:

12352204

NACRES:

NA.54

Numéro CE :

3.6.1.5 (BRENDA, IUBMB)

MDL number:

MFCD00130542

Biological source:

potato

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Propriétés

biological source

potato

form

lyophilized powder

quality

High Activity

ATPase activity

≥600 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

protein, ≥30%

foreign activity

Acid Phosphatase ≤2% of base activity

shipped in

wet ice

storage temp.

−20°C

Description

Application

Au moins deux isoenzymes sont présentes dans diverses souches de S. tuberosum : l′une avec un rapport ATPase/ADPase élevé (∼ 10) et l′autre avec un rapport faible (∼ 1).
Réaction : ATP → ADP+P_i → AMP+2P_i.

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation [1]. Product A2230 is a high activity apyrase.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis [2]. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Physical form

Lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

Other Notes

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Comparer avec des articles similaires

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Cet article	A6132	A6237	SRE0019
Sigma-Aldrich A2230 Apyrase from potatoes	Sigma-Aldrich A6132 Apyrase from potatoes	Sigma-Aldrich A6237 Apyrase from potato	Sigma-Aldrich SRE0019 Apyrase from potatoes
description High Activity, ATPase ≥600 units/mg protein, lyophilized powder	description ATPase ≥3.0 units/mg protein, lyophilized powder	description recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder	description Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein
biological source potato	biological source potato	biological source -	biological source -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in wet ice	shipped in -
foreign activity Acid Phosphatase ≤2% of base activity	foreign activity -	foreign activity -	foreign activity Acid Phosphatase ≤2% of base activity
quality High Activity	quality -	quality -	quality -

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c