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Anti-Thioredoxin–Agarose antibody produced in rabbit

Name: Anti-Thioredoxin–Agarose antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Thioredoxin

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

agarose conjugate

Clone:

polyclonal

Application:

IP, affinity chromatography

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

rabbit

Quality Segment

200

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

mol wt

antigen 11.7 kDa

species reactivity

E. coli

analyte chemical class(es)

proteins (thioredoxin)

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Thioredoxin is a small electron transport protein. The thioredoxin system consists of thioredoxin, thioredoxin-reductase and NADPH. Thioredoxin obtained from E. coli is made up of a single polypeptide chain of 108 amino acids. It has a molecular weight of 11,700. The protein contains no prosthetic group or bound metals.

Immunogen

recombinant E. coli thioredoxin.

Application

Anti-Thioredoxin−Agarose antibody produced in rabbit has been used for immunoaffinity purification and immunoprecipitation of thioredoxin from bacterial lysates.

Immunofluorescence was carried out on the cerivcal cancer cell lines SiHa, CaSki, and HeLa using an antibody against the redox proteinThioredoxin.

Biochem/physiol Actions

Specific for natural E. coli and recombinant thioredoxin. It may be used to identify and purify the expression of thioredoxin fusion proteins.

Thioredoxin is a 11.7kDa electron transport protein that functions as a hydrogen donor during the enzymatic reduction of ribonucleotides to yield deoxyribonucleotides. The thioredoxin system is also involved in the enzymatic reductions of methionine sulfoxide and sulfate. The gene encoding E. coli thioredoxin has been cloned into several plasmids and serves as a fusion partner for the expression of other cloned genes. Hence, the system is useful for the production of soluble fusion proteins in the E. coli cytoplasm. Furthermore, in several instances, these fusion proteins fold correctly and thus display full biological activity.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Prepared using antibody coupled to cyanogen bromide activated agarose at 5 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	T0803	A1806	G6532
$Anti-Thioredoxin–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension$ Sigma-Aldrich A2582 Anti-Thioredoxin–Agarose antibody produced in rabbit	$Anti-Thioredoxin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich T0803 Anti-Thioredoxin antibody produced in rabbit	Sigma-Aldrich A1806 Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse	$Anti-GroEL antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich G6532 Anti-GroEL antibody produced in rabbit
description IgG fraction of antiserum, PBS suspension	description IgG fraction of antiserum, buffered aqueous solution	description clone PT-66, purified immunoglobulin, PBS solution	description IgG fraction of antiserum, buffered aqueous solution
conjugate agarose conjugate	conjugate unconjugated	conjugate agarose conjugate	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form purified immunoglobulin	antibody form IgG fraction of antiserum
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
clone polyclonal	clone polyclonal	clone PT-66, monoclonal	clone polyclonal
form PBS suspension	form buffered aqueous solution	form PBS solution	form buffered aqueous solution

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