This product is a conjugate of 5'-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5'-ATP. A 9-atom matrix spacer arm connects the ATP and the agarose. Please see the link below to review the product datasheet for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/174/887/a2767pis.pdf
A2767
Adenosine 5′-triphosphate–Agarose
lyophilized powder
Synonyme(s) :
5′-ATP agarose
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1 mL | Vérifier la disponibilité des articles du panier | 142.00 CHF |
5 mL | Vérifier la disponibilité des articles du panier | 546.00 CHF |
25 mL | Vérifier la disponibilité des articles du panier | 2 320.00 CHF |
A propos de cet article
Biological source:
plant (Sea weed)
Form:
lyophilized powder
Storage temp.:
−20°C
142.00 CHF
Vérifier la disponibilité des articles du panier
Service technique
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Laissez-nous vous aiderbiological source
plant (Sea weed)
Quality Segment
form
lyophilized powder
extent of labeling
1-5 μmol per mL
matrix
cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
C-8
matrix spacer
9 atoms
storage temp.
−20°C
SMILES string
[X]Nc1ncnc2[n](cnc21)[C@@H]3O[C@@H]([C@H]([C@H]3O)O)CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O
General description
Adenosine 5′-triphosphate–Agarose (5′-ATP-agarose) is a conjugate of 5′-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5′-ATP. 5′-ATP–agarose is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins, and cryptochromes.
Application
Adenosine 5′-triphosphate Agarose (5′-ATP agarose) has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.
Adenosine 5′-triphosphate–Agarose (5′-ATP–agarose) is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins-70 (HSP-70), and cryptochromes. 5′-ATP agarose has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.
Physical form
Lyophilized powder stabilized with lactose
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Cet article | |||
|---|---|---|---|
| description lyophilized powder | description aqueous glycerol suspension | description lyophilized powder | description suitable for affinity chromatography, powder (lyophilized) |
| biological source plant (Sea weed) | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form aqueous glycerol suspension | form lyophilized powder | form powder (lyophilized) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| extent of labeling 1-5 μmol per mL | extent of labeling ≥1 μmol per mL | extent of labeling 1-5 μmol per mL | extent of labeling - |
| matrix attachment C-8 | matrix attachment ribose hydroxyls | matrix attachment C-8 | matrix attachment - |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Where is the linker attached on the ATP molecule
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How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?
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The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes. Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin. Do not allow the resin to dry. Resuspend the resin in excess water or starting buffer to pack the column bed.
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How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?
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Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl. Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.
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Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?
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For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C. In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage. The resin can be regenerated; please see the regeneration FAQ for this product.
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How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?
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Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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