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Anti-Atg16L antibody produced in rabbit

Name: Anti-Atg16L antibody produced in rabbit
Brand: SIGMA

fractionated antiserum, buffered aqueous solution

Synonym(s):

Anti-APG16L, Anti-ATG16 autophagy related 16-like 1 (S. cerevisiae), Anti-ATG16L1

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

rabbit

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa (several isonform bands)

species reactivity

rat, mouse, human

technique(s)

western blot: 1:500-1:1,000 using whole extracts of human A549 cells, rat PC12 cells, and HEK-293T cells expressing mouse Atg16l

UniProt accession no.

Q676U5

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG16L1(55054)
mouse ... Atg16l1(77040)
rat ... Atg16l1(363278)

Description

General description

ATG16L is a component of a large protein complex that is critical for autophagy.

Autophagy-related 16-like 1 (ATG16L1) is an autophagy gene, that is expressed in the colon, small intestine, intestinal epithelial cells, leukocytes and spleen. Atg16L is expressed in different isoform patterns depending on the tissue.

Immunogen

synthetic peptide correspopnding to amino acids 2-15 of mouse Atg16l, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and human.

Application

Anti-Atg16L antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Atg16L is involved in LC3 lipidation during the formation of autphagosomes. Studies have reported that Atg16L may also function as a mammalian Rab33 effector.

Autophagy-related 16-like 1 (ATG16L1) modulates host immune responses and is linked with several diseases, like cardiovascular disease (CVD). Atg16 multimeric complex plays an essential role in autophagy. Atg16L interacts with both Atg5 and additional Atg16L monomers. Together with Atg12-Atg5 conjugate, Atg16L associates with the autophagic isolation membrane for the duration of autophagosome formation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A2981	SAB4200100	HPA050312
$Anti-Atg16L antibody produced in rabbit fractionated antiserum, buffered aqueous solution$ Sigma-Aldrich A7356 Anti-Atg16L antibody produced in rabbit	$Anti-ATG4B antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A2981 Anti-ATG4B antibody produced in rabbit	Sigma-Aldrich SAB4200100 Anti-ATG13 antibody produced in rabbit	Sigma-Aldrich HPA050312 Anti-ATG16L2 antibody produced in rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
antibody form fractionated antiserum	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
species reactivity rat, mouse, human	species reactivity mouse, rat, human	species reactivity human, rat, mouse	species reactivity human
mol wt antigen ~70 kDa (several isonform bands)	mol wt antigen ~44 kDa	mol wt antigen ~65 kDa	mol wt -
technique(s) western blot: 1:500-1:1,000 using whole extracts of human A549 cells, rat PC12 cells, and HEK-293T cells expressing mouse Atg16l	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtaAvidin^®-Peroxidase staining of heat-retrieved human cerebellum sections, western blot: 1:125-1:500 using whole extract of 3T3 cells, western blot: 1:500-1:1,000 using whole extract of rat brain	technique(s) western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.	technique(s) immunofluorescence: 0.25-2 μg/mL

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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